Invitrogen

CBP Polyclonal Antibody

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CBP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CREB Binding Protein (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

CBP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CREB Binding Protein showing staining in the cytoplasm and nucleus of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) diluted in 3% BSA-PBS at a dilution of 1:2000 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

CBP Antibody in ChIP assay (ChIP) — Chromatin immunoprecipitation analysis of CREB Binding Protein was performed using cross-linked chromatin from 1x10^6 HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a CREB Binding Protein polyclonal antibody (Product # PA1-847). Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

CBP Antibody in Immunohistochemistry (IHC) — Published figure using CBP polyclonal antibody (Product # PA1-847) in Immunohistochemistry

CBP Antibody in Western Blot (WB) — Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Product Details

PA1-847

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000-1:5,000	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
ChIP assay (ChIP)	1-3 µL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2083939

Product Specific Information

PA1-847 detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) from human and mouse tissues and cells.

PA1-847 has been successfully used in ChIP, ICC/IF, Western blot and IHC-P procedures. By Western blot, this antibody detects an ~265 kDa protein representing CBP and a larger, unknown protein at ~400 kDa from HeLa cell lysate.

The PA1-847 immunogen is a synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. The PA1-847 immunizing peptide (Cat. # PEP-052) is available for use in neutralization and control experiments.

Target Information

CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Purine-Induced IFN-γ Promotes Uric Acid Production by Upregulating Xanthine Oxidoreductase Expression.

Frontiers in immunology

Wang H,Xie L,Song X,Wang J,Li X,Lin Z,Su T,Liang B,Huang D

PA1-847 was used in ChIP assay to explore whether exogenous purines are responsible for increased xanthine oxidoreductase expression and activity.

Thu Mar 03 00:00:00 EST 2022

Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia.

eLife

Takao S,Forbes L,Uni M,Cheng S,Pineda JMB,Tarumoto Y,Cifani P,Minuesa G,Chen C,Kharas MG,Bradley RK,Vakoc CR,Koche RP,Kentsis A

PA1-847 was used in Western Blot to conclude that aberrant assembly and sequestration of MYB:CBP/P300 complexes provide a unifying mechanism of oncogenic gene expression in AML.

Tue Feb 02 00:00:00 EST 2021

Aberrant activation of CYR61 enhancers in colorectal cancer development.

Journal of experimental & clinical cancer research : CR

Xie L,Song X,Lin H,Chen Z,Li Q,Guo T,Xu T,Su T,Xu M,Chang X,Wang LK,Liang B,Huang D

PA1-847 was used in Western Blotting to study the mechanism of action of cysteine-rich 61 in the regulation of transcription in colorectal cancer.

Wed May 22 00:00:00 EDT 2019

Peptidomimetic blockade of MYB in acute myeloid leukemia.

Nature communications

Ramaswamy K,Forbes L,Minuesa G,Gindin T,Brown F,Kharas MG,Krivtsov AV,Armstrong SA,Still E,de Stanchina E,Knoechel B,Koche R,Kentsis A

Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Tue Jan 09 00:00:00 EST 2018

Expression of glial CBP in steroid mediated neuroprotection in male and female zebra finches.

Journal of chemical neuroanatomy

Klores M,Moon JT,Duncan KA

PA1847 was used in immunohistochemistry to explore the role of cAMP response element-binding protein-binding protein in the estrogen and immune response to injury

Sun Jan 01 00:00:00 EST 2017

Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2.

Molecular endocrinology (Baltimore, Md.)

Hahm JB,Privalsky ML

PA1-847 was used in Western Blotting to identify a series of previously unrecognized proteins that selectively interact with thyroid hormone receptorΒ2, compared with the more widely expressed TRΒ1.

Wed May 01 00:00:00 EDT 2013

Cells in behaviourally relevant brain regions coexpress nuclear receptor coactivators and ovarian steroid receptors.

Journal of neuroendocrinology

Tetel MJ,Siegal NK,Murphy SD

Published figure using CBP polyclonal antibody (Product # PA1-847) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2007

Cells in behaviourally relevant brain regions coexpress nuclear receptor coactivators and ovarian steroid receptors.

Journal of neuroendocrinology

Tetel MJ,Siegal NK,Murphy SD

Published figure using CBP polyclonal antibody (Product # PA1-847) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2007

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats.

Hormones and behavior

Molenda-Figueira HA,Williams CA,Griffin AL,Rutledge EM,Blaustein JD,Tetel MJ

Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Fri Sep 01 00:00:00 EDT 2006

Nuclear receptor coactivators modulate hormone-dependent gene expression in brain and female reproductive behavior in rats.

Endocrinology

Molenda HA,Griffin AL,Auger AP,McCarthy MM,Tetel MJ

PA1-847 was used in immunocytochemistry to demonstrate the regulatory role of nuclear receptors in ER transcriptional activity in brain and the expression of hormone-dependent behavior.

Fri Feb 01 00:00:00 EST 2002

The transcriptional coactivators p300 and CBP are histone acetyltransferases.

Cell

Ogryzko VV,Schiltz RL,Russanova V,Howard BH,Nakatani Y

PA1-847 was used in western blot to investigate the functional properties of CREB binding protein and p300

Fri Nov 29 00:00:00 EST 1996

Bioinformatics

Protein Aliases: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein-lysine acetyltransferase CREBBP

Gene Aliases: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS

UniProt ID: (Human) Q92793

Entrez Gene ID: (Human) 1387, (Mouse) 12914

Molecular Function: histone modifying enzyme

Function(s)

negative regulation of transcription from RNA polymerase II promoter response to hypoxia stimulatory C-type lectin receptor signaling pathway regulation of transcription, DNA-templated transcription initiation from RNA polymerase II promoter protein complex assembly protein acetylation signal transduction Notch signaling pathway cell proliferation regulation of smoothened signaling pathway viral process histone acetylation N-terminal peptidyl-lysine acetylation positive regulation of type I interferon production cellular response to UV homeostatic process embryonic digit morphogenesis regulation of apoptotic process cellular lipid metabolic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of cell adhesion molecule production regulation of transcription from RNA polymerase II promoter in response to hypoxia response to interleukin-1 cell-cell adhesion regulation of cellular response to heat positive regulation of G1/S transition of mitotic cell cycle positive regulation of NIK/NF-kappaB signaling beta-catenin-TCF complex assembly transcription, DNA-templated transcription from RNA polymerase II promoter germ-line stem cell population maintenance positive regulation of sequence-specific DNA binding transcription factor activity

Process(es)

core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcriptional repressor activity, RNA polymerase II transcription factor binding p53 binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity histone acetyltransferase activity signal transducer activity protein binding transcription factor binding zinc ion binding acetyltransferase activity peptide N-acetyltransferase activity peroxisome proliferator activated receptor binding MRF binding SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription cofactor activity transferase activity transferase activity, transferring acyl groups activating transcription factor binding metal ion binding

CBP Polyclonal Antibody

Product Details

PA1-847

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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