Invitrogen

CBP Polyclonal Antibody

CBP Antibody in Immunocytochemistry (ICC/IF)

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CBP Antibody (PA5-27369) in ICC/IF

Immunofluorescence analysis of CBP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CBP Polyclonal Antibody (Product # PA5-27369) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution... View More

CBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CBP Antibody in Immunoprecipitation (IP)

CBP Antibody in Western Blot, Immunocytochemistry, ChIP Assay (WB, ICC/IF, ChIP)

Product Image Gallery

CBP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with CBP siRNA and reduction of specific signal was observed in immunofluorescence application using CBP Polyclonal antibody (Product # PA5-27369). {KD}

Product Details

PA5-27369

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

View 2 publications 2 publications

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Non-human primate, Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 2123 and 2442 of Human CBP

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.82 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544845

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT-116, Vero E6.

Predicted reactivity: Mouse (94%), Rat (94%), Rhesus Monkey (100%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pharmacological targeting of Sam68 functions in colorectal cancer stem cells.

iScience

Masibag AN,Bergin CJ,Haebe JR,Zouggar A,Shah MS,Sandouka T,Mendes da Silva A,Desrochers FM,Fournier-Morin A,Benoit YD

Published figure using CBP polyclonal antibody (Product # PA5-27369) in Western Blot

Fri Dec 17 00:00:00 UTC 2021

Pharmacological targeting of Sam68 functions in colorectal cancer stem cells.

iScience

Masibag AN,Bergin CJ,Haebe JR,Zouggar A,Shah MS,Sandouka T,Mendes da Silva A,Desrochers FM,Fournier-Morin A,Benoit YD

Published figure using CBP polyclonal antibody (Product # PA5-27369) in Western Blot

Fri Dec 17 00:00:00 UTC 2021

PER2 mediates CREB-dependent light induction of the clock gene Per1.

Scientific reports

Brenna A,Ripperger JA,Saro G,Glauser DA,Yang Z,Albrecht U

Published figure using CBP polyclonal antibody (Product # PA5-27369) in ChIP assay

Fri Nov 05 00:00:00 UTC 2021

PER2 mediates CREB-dependent light induction of the clock gene Per1.

Scientific reports

Brenna A,Ripperger JA,Saro G,Glauser DA,Yang Z,Albrecht U

Published figure using CBP polyclonal antibody (Product # PA5-27369) in ChIP assay

Fri Nov 05 00:00:00 UTC 2021

PER2 mediates CREB-dependent light induction of the clock gene Per1.

Scientific reports

Brenna A,Ripperger JA,Saro G,Glauser DA,Yang Z,Albrecht U

Published figure using CBP polyclonal antibody (Product # PA5-27369) in ChIP assay

Fri Nov 05 00:00:00 UTC 2021

PER2 mediates CREB-dependent light induction of the clock gene Per1.

Scientific reports

Brenna A,Ripperger JA,Saro G,Glauser DA,Yang Z,Albrecht U

Published figure using CBP polyclonal antibody (Product # PA5-27369) in ChIP assay

Fri Nov 05 00:00:00 UTC 2021

PER2 mediates CREB-dependent light induction of the clock gene Per1.

Scientific reports

Brenna A,Ripperger JA,Saro G,Glauser DA,Yang Z,Albrecht U

Published figure using CBP polyclonal antibody (Product # PA5-27369) in ChIP assay

Fri Nov 05 00:00:00 UTC 2021

Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription.

Journal of virology

Martella C,Tollenaere AI,Waast L,Lacombe B,Groussaud D,Margottin-Goguet F,Ramirez BC,Pique C

Published figure using CBP polyclonal antibody (Product # PA5-27369) in Western Blot

Tue Mar 31 00:00:00 UTC 2020

Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription.

Journal of virology

Martella C,Tollenaere AI,Waast L,Lacombe B,Groussaud D,Margottin-Goguet F,Ramirez BC,Pique C

Published figure using CBP polyclonal antibody (Product # PA5-27369) in Western Blot

Tue Mar 31 00:00:00 UTC 2020

Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells.

Cancers

Castellaro AM,Rodriguez-Baili MC,Di Tada CE,Gil GA

PA5-27369 was used in Immunoprecipitation to explore the mechanisms behind macrophage-mediated induction of endocrine resistance in ER+ breast cancer cells.

Wed Feb 06 00:00:00 UTC 2019

DYRK1A interacts with histone acetyl transferase p300 and CBP and localizes to enhancers.

Nucleic acids research

Li S,Xu C,Fu Y,Lei PJ,Yao Y,Yang W,Zhang Y,Washburn MP,Florens L,Jaiswal M,Wu M,Mohan M

Published figure using CBP polyclonal antibody (Product # PA5-27369) in ChIP assay

Fri Nov 30 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein lactyltransferas CREBBP; Protein-lysine acetyltransferase CREBBP

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Gene Aliases: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS

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UniProt ID: (Human) Q92793

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Entrez Gene ID: (Human) 1387, (Mouse) 12914

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Function(s)

core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcriptional repressor activity, RNA polymerase II transcription factor binding p53 binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity histone acetyltransferase activity signal transducer activity protein binding transcription factor binding zinc ion binding acetyltransferase activity peptide N-acetyltransferase activity peroxisome proliferator activated receptor binding MRF binding SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription cofactor activity transferase activity transferase activity, transferring acyl groups activating transcription factor binding metal ion binding histone modifying enzyme

Process(es)

negative regulation of transcription from RNA polymerase II promoter response to hypoxia stimulatory C-type lectin receptor signaling pathway regulation of transcription, DNA-templated transcription initiation from RNA polymerase II promoter protein complex assembly protein acetylation signal transduction Notch signaling pathway cell proliferation regulation of smoothened signaling pathway viral process histone acetylation N-terminal peptidyl-lysine acetylation positive regulation of type I interferon production cellular response to UV homeostatic process embryonic digit morphogenesis regulation of apoptotic process cellular lipid metabolic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of cell adhesion molecule production regulation of transcription from RNA polymerase II promoter in response to hypoxia response to interleukin-1 cell-cell adhesion regulation of cellular response to heat positive regulation of G1/S transition of mitotic cell cycle positive regulation of NIK/NF-kappaB signaling beta-catenin-TCF complex assembly transcription, DNA-templated transcription from RNA polymerase II promoter germ-line stem cell population maintenance positive regulation of sequence-specific DNA binding transcription factor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CBP Polyclonal Antibody

CBP Antibody (PA5-27369) in ICC/IF

Product Details

PA5-27369

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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