Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
CBP for CREB-binding protein is a 265kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.
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Protein Aliases: CREB-binding protein; KAT3A; RSTS
Gene Aliases: CBP; CREBBP; KAT3A; RSTS
UniProt ID: (Human) Q92793
Entrez Gene ID: (Human) 1387