Proteintech

CBP/EIF4E Polyclonal Antibody

CBP/EIF4E Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

CBP/EIF4E Antibody (11149-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using EIF4E antibody 11149-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

CBP/EIF4E Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CBP/EIF4E Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

11149-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CBP,EIF4E Fusion Protein Ag1626 (1-217 aa encoded by BC012611)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against the full-length human EIF4E protein. This protein can form a dimer (~50 kDa).

Immunogen sequence: MATVEPETT PTPNPPTTEE EKTESNQEVA NPEHYIKHPL QNRWALWFFK NDKSKTWQAN LRLISKFDTV EDFWALYNHI QLSSNLMPGC DYSLFKDGIE PMWEDEKNKR GGRWLITLNK QQRRSDLNRF WLETLLCLIG ESFDDYSDDV CGAVVNVRAK GDKIAIWTTE CENREAVTHI GRVYKERLGL PPKIVIGYQS HADTATKSGS TTKNRFVV (1-217 aa encoded by BC012611)

Target Information

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Thu Mar 30 00:00:00 UTC 2023

N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma.

European journal of medical research

Maimaiti A,Feng Z,Liu Y,Turhon M,Xie Z,Baihetiyaer Y,Wang X,Kasimu M,Jiang L,Wang Y,Wang Z,Pei Y

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TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury.

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RNA m⁶A methylation regulates dissemination of cancer cells by modulating expression and membrane localization of β-catenin.

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Du Y,Hou G,Zhang H,Dou J,He J,Guo Y,Li L,Chen R,Wang Y,Deng R,Huang J,Jiang B,Xu M,Cheng J,Chen GQ,Zhao X,Yu J

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SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

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International journal of clinical and experimental medicine

Zhao L,Teng B,Wen L,Feng Q,Wang H,Li N,Wang Y,Liang Z

Thu Mar 06 00:00:00 UTC 2014

mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma.

International journal of clinical and experimental medicine

Zhao L,Teng B,Wen L,Feng Q,Wang H,Li N,Wang Y,Liang Z

Thu Mar 06 00:00:00 UTC 2014

mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma.

International journal of clinical and experimental medicine

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Bioinformatics

Protein Aliases: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705

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Gene Aliases: AUTS19; CBP; eIF-4E; EIF4E; EIF4E1; EIF4EL1; EIF4F

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UniProt ID: (Human) P06730

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Entrez Gene ID: (Human) 1977

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Function(s)

RNA cap binding translation initiation factor activity protein binding enzyme binding eukaryotic initiation factor 4G binding poly(A) RNA binding repressing transcription factor binding translation factor translation initiation factor translational protein

Process(es)

G1/S transition of mitotic cell cycle nuclear-transcribed mRNA poly(A) tail shortening behavioral fear response RNA export from nucleus mRNA export from nucleus translational initiation regulation of translation viral process negative regulation of translation stem cell population maintenance lung development negative regulation of neuron differentiation positive regulation of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CBP/EIF4E Polyclonal Antibody

CBP/EIF4E Antibody (11149-1-AP) in ICC/IF

Product Details

11149-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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