Invitrogen

CBS Polyclonal Antibody

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CBS Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of CBS was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: CBS Polyclonal Antibody (Product # PA5-22273) diluted at 1:200. Blue: Hoechst 33342 staining.

CBS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS (Product # PA5-22273) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

CBS Antibody in Western Blot (WB) — Western Blot analysis of CBS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CBS Polyclonal Antibody (Product # PA5-22273) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

CBS Antibody in Immunoprecipitation (IP) — CBS Polyclonal Antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 µg HepG2 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of CBS protein by 4 µg CBS Polyclonal Antibody (Product # PA5-22273). 7.5 % SDS-PAGE. The immunoprecipitated CBS protein was detected by CBS Polyclonal Antibody (Product # PA5-22273) diluted at 1:500.

Product Details

PA5-22273

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 39 and 251 of CBS (Uniprot ID#P35520) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.81 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11154123

Product Specific Information

Recommended positive controls: HeLa, 293T, mouse liver.

Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (93%), Rabbit (95%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cystathionine metabolic enzymes play a role in the inflammation resolution of human keratinocytes in response to sub-cytotoxic formaldehyde exposure.

Toxicology and applied pharmacology

Lee E,Kim HJ,Lee M,Jin SH,Hong SH,Ahn S,Kim SO,Shin DW,Lee ST,Noh M

PA522273 was used in western blot to assess the effects of sub-cytotoxic exposure to formaldehyde on skin cells

Tue Nov 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; CBSL; HIP4

UniProt ID: (Human) P35520, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Mouse) 12411

Molecular Function: lyase metabolite interconversion enzyme