Invitrogen

CBS Polyclonal Antibody

Product Image Gallery

CBS Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CBS in paraformaldehyde-fixed HeLa cells using a CBS polyclonal antibody (Product # PA5-22273) at a 1:200 dilution.

CBS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CBS in paraffin-embedded OVCAR3 xenograft using a CBS polyclonal antibody (Product # PA5-22273) at a dilution of 1:500.

CBS Antibody in Western Blot (WB) — Western blot analysis of CBS using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CBS polyclonal antibody (Product # PA5-22273) at a dilution of 1:5000.

CBS Antibody in Immunoprecipitation (IP) — PA5-22273 immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 µg HepG2 whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 µg CBS antibody (Product # PA5-22273) on 7.5% SDS-PAGE.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

1:500-1:3000

Published Applications

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 39 and 251 of CBS (Uniprot ID#P35520)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154123

Product Specific Information

PA5-22273 targets CBS in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-22273 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 251 (P35520) of CBS.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; HIP4; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: CBS; CBSL; HIP4

UniProt ID: (Human) P0DN79

Entrez Gene ID: (Human) 875

Molecular Function: deaminase dehydratase epimerase/racemase hydrolase isomerase lyase