Invitrogen

CBS Polyclonal Antibody

Product Image Gallery

CBS Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of CBS was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: CBS Polyclonal Antibody (Product # PA5-22273) diluted at 1:200. Blue: Hoechst 33342 staining.

CBS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CBS in paraffin-embedded OVCAR3 xenograft using a CBS polyclonal antibody (Product # PA5-22273) at a dilution of 1:500.

CBS Antibody in Western Blot (WB) — Western Blot analysis of CBS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CBS Polyclonal Antibody (Product # PA5-22273) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

CBS Antibody in Immunoprecipitation (IP) — PA5-22273 immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 µg HepG2 whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 µg CBS antibody (Product # PA5-22273) on 7.5% SDS-PAGE.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 39 and 251 of CBS (Uniprot ID#P35520)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154123

Product Specific Information

Recommended positive controls: HeLa, 293T, mouse liver.

Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (93%), Rabbit (95%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; CBSL; HIP4

UniProt ID: (Human) P0DN79, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Mouse) 12411

Molecular Function: deaminase dehydratase epimerase/racemase hydrolase isomerase lyase