Invitrogen

CBS Polyclonal Antibody

View all (23) CBS antibodies

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Antibody Testing Data (4)

CBS Antibody in Western Blot (WB)

FIGURE: 1 / 4

CBS Antibody (PA5-72505) in WB

Western blot analysis of CBS in mouse kidney tissue and Ramos cell lysate using 35 µg of protein per lane. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505).

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CBS Antibody in Western Blot (WB) — Western blot analysis of CBS in mouse kidney tissue and Ramos cell lysate using 35 µg of protein per lane. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505).

CBS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CBS in paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a peroxidase-conjugate secondary antibody and DAB staining.

CBS Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CBS in Ramos cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a FITC conjugated goat anti-rabbit secondary antibody.

CBS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CBS in 293 cells using confocal microscopy. Samples were treated with CBS H1 polyclonal antibody (Product # PA5-72505). Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used as a secondary antibody. Actin filaments were labeled with Alexa Fluor 555 Phalloidin (red) and DAPI was used to stain the cell nuclei (blue).

Product Details

PA5-72505

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:10-1:50

-

Flow Cytometry (Flow)

1:10-1:50

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human CBS

Conjugate

Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2718359

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; HIP4

UniProt ID: (Human) P35520, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Mouse) 12411

Molecular Function: lyase metabolite interconversion enzyme

cysteine biosynthetic process from serine L-serine metabolic process L-serine catabolic process cellular amino acid biosynthetic process cysteine biosynthetic process via cystathionine cysteine biosynthetic process transsulfuration L-cysteine catabolic process DNA protection homocysteine catabolic process homocysteine metabolic process oxidation-reduction process hydrogen sulfide biosynthetic process endochondral ossification blood vessel remodeling superoxide metabolic process cerebellum morphogenesis regulation of cGMP metabolic process negative regulation of apoptotic process regulation of JUN kinase activity regulation of blood vessel size response to folic acid maternal process involved in female pregnancy cartilage development involved in endochondral bone morphogenesis cellular response to hypoxia

Process(es)

cystathionine beta-synthase activity cysteine synthase activity protein binding oxygen binding enzyme binding heme binding pyridoxal phosphate binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity metal ion binding nitrite reductase (NO-forming) activity carbon monoxide binding nitric oxide binding modified amino acid binding S-adenosyl-L-methionine binding lyase activity