Invitrogen

CBS Polyclonal Antibody

Product Image Gallery

CBS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CBS in 293 cells using confocal microscopy. Samples were treated with CBS H1 polyclonal antibody (Product # PA5-72505). Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used as a secondary antibody. Actin filaments were labeled with Alexa Fluor 555 Phalloidin (red) and DAPI was used to stain the cell nuclei (blue).

CBS Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of CBS in paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a peroxidase-conjugate secondary antibody and DAB staining.

CBS Antibody in Western Blot (WB) — Western blot analysis of CBS in mouse kidney tissue and Ramos cell lysate using 35 µg of protein per lane. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505).

CBS Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CBS in Ramos cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a FITC conjugated goat anti-rabbit secondary antibody.

Product Details

PA5-72505

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Immunocytochemistry (ICC/IF)	1:10-1:50	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human CBS View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2718359

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; CBSL; HIP4

UniProt ID: (Human) P35520, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Mouse) 12411

Molecular Function: lyase metabolite interconversion enzyme