Invitrogen

CBS Polyclonal Antibody

Product Image Gallery

CBS Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CBS in 293 cells using confocal microscopy. Samples were treated with CBS H1 polyclonal antibody (Product # PA5-72505). Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used as a secondary antibody. Actin filaments were labeled with Alexa Fluor 555 Phalloidin (red) and DAPI was used to stain the cell nuclei (blue).

CBS Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of CBS in paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a peroxidase-conjugate secondary antibody and DAB staining.

CBS Antibody in Western Blot (WB) — Western blot analysis of CBS in mouse kidney tissue and Ramos cell lysate using 35 µg of protein per lane. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505).

CBS Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CBS in Ramos cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a FITC conjugated goat anti-rabbit secondary antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:10-1:50

Immunocytochemistry (ICC)

1:10-1:50

Immunofluorescence (IF)

1:10-1:50

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human CBS

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2718359

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury .

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; HIP4; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; CBSL; HIP4

UniProt ID: (Human) P0DN79, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Mouse) 12411

Molecular Function: deaminase dehydratase epimerase/racemase hydrolase isomerase lyase