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CBX4 Antibody (PA5-96352) in WB
Western blot analysis of CBX4 in extracts of various cell lines. Samples were incubated with CBX4 Polyclonal antibody (Product # PA5-96352) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-96352
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_0036462)
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808154
Product Specific Information
Immunogen sequence: AVGLNLSHVR KRCLSETHGE REPCKKRLTA RSISTPTCLG GSPAAERPAD LPPAAALPQP EVILLDSDLD EPIDLRCVKT RSEAGEPPSS LQVKPETPAS AAVAVAAAAA PTTTAEKPPA EAQDEPAESL SEFKPFFGNI IITDVTANCL TVTFKEYVTV; Positive Samples: MCF-7, HepG2, SW620, Jurkat, Mouse brain, Mouse liver, Rat liver; Cellular Location: Nucleus, Nucleus speckle
Target Information
Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chromobox homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox protein homolog 4; chromobox-like protein 4; E3 SUMO-protein ligase CBX4; E3 SUMO-protein transferase CBX4; hPc2; NS5ATP1-binding protein 16; Pc class 2 homolog; Pc2; Polycomb 2 homolog
Gene Aliases: CBX4; MPc2; NBP16; PC2
UniProt ID: (Human) O00257, (Mouse) O55187
Entrez Gene ID: (Human) 8535, (Mouse) 12418, (Rat) 501403
chromatin binding
transcription corepressor activity
single-stranded RNA binding
protein binding
ligase activity
SUMO transferase activity
enzyme binding
SUMO binding
methylated histone binding
transcription regulatory region DNA binding
phosphoprotein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
covalent chromatin modification
protein sumoylation
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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