Invitrogen

CCKBR Polyclonal Antibody

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CCKBR Antibody in Western Blot (WB) — Western blot analysis of CCKBR was performed by separating 30 µg of unboiled HeLa cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CCKBR Polyclonal Antibody (Product # PA5-27169) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

CCKBR Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of CCK-BR in the cytosol of human lung carcinoma using a CCK-BR polyclonal antibody (Product # PA5-27169) at a dilution of 1:250.

CCKBR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CCK-BR in the membrane of paraffin-embedded colon carcinoma using a CCK-BR polyclonal antibody (Product # PA5-27169) at a dilution of 1:500.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 271 and 363 of Human CCK-BR

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544645

Product Specific Information

Recommended positive controls: Unboiled HeLa cell extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CCKBR is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. CCKBR has been reported in brain, colon, pancreas, stomach, thyroid, and various cancers.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CCK-B receptor; CCK-BR; CCK2 receptor; CCK2-R; CCKB; Cckb receptor; CCKB-R; Cholecystokinin-2 receptor; Cholecystokinin-B; gastrin receptor; Gastrin/cholecystokinin type B receptor; HGNC:1571

Gene Aliases: CCK-B; CCK2R; CCKBR; CCKRB; GASR

UniProt ID: (Human) P32239

Entrez Gene ID: (Human) 887

Molecular Function: G-protein coupled receptor receptor