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Invitrogen
CCL19 Polyclonal Antibody
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FIGURE: 1 / 1
CCL19 Antibody (PA5-78936) in WB
Western blot analysis of CCL19 in Lane 1: rat thymus tissue lysate, Lane 2: rat lung tissue lysate, Lane 3: rat spleen tissue lysate, Lane 4: rat stomach tissue lysate, Lane 5: rat PC-12 whole cell lysate, Lane 6: mouse thymus tissue lysate, Lane 7: mouse NIH3T3 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5... View More
Product Details
PA5-78936
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat Ccl19/MIP-3 beta recombinant protein (Position: G26-S108).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat thymus tissue, rat lung tissue, rat spleen tissue, rat stomach tissue, rat PC-12 whole cell, mouse thymus tissue, mouse NIH3T3 whole cell.
Target Information
CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine; C-C motif chemokine 19; chemokine CCL19; EBI-1 ligand chemokine; EBI1 ligand chemokine; EBI1-ligand chemokine; EBV-induced molecule 1 ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; M-MIP-3-beta; small inducible cytokine A19; Small-inducible cytokine A19
Gene Aliases: Ccl19; CKb11; ELC; exodus-3; MIP3B; Scya19
UniProt ID: (Mouse) O70460
Entrez Gene ID: (Mouse) 24047, (Rat) 362506
establishment of T cell polarity
dendritic cell chemotaxis
myeloid dendritic cell chemotaxis
response to prostaglandin E
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of protein kinase activity
positive regulation of JNK cascade
release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
response to nitric oxide
positive regulation of neutrophil chemotaxis
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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