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Invitrogen
CCL20 Polyclonal Antibody
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FIGURE: 1 / 1
CCL20 Antibody (PA5-95917) in WB
Western blot analysis of CCL20 in extracts of various cell lines. Samples were incubated with CCL20 Polyclonal antibody (Product # PA5-95917) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5 min.
Product Details
PA5-95917
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-95 of human CCL20 (NP_0045821)
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: K-562, LO2, A-549, BxPC-3
Immunogen sequence: MCCTKSLLLA ALMSVLLLHL CGESEAASNF DCCLGYTDRI LHPKFIVGFT RQLANEGCDI NAIIFHTKKK LSVCANPKQT WVKYIVRLLS KKVKN
Target Information
Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta chemokine exodus-1; Beta-chemokine exodus-1; C-C motif chemokine 20; CC chemokine LARC; chemokine (C-C motif) ligand 20; Liver and activation-regulated chemokine; M-MIP-3-alpha; Macrophage inflammatory protein 3 alpha; MIP 3 a; MIP 3 α; MIP 3alpha; MIP 3α; MIP-3-alpha; MIP3 a; MIP3 alpha; MIP3 α; small inducible cytokine subfamily A (Cys-Cys), member 20; Small-inducible cytokine A20
Gene Aliases: CCL20; CKb4; Exodus; LARC; MIP-3-alpha; MIP-3a; MIP3A; SCYA20; ST38
UniProt ID: (Human) P78556
Entrez Gene ID: (Human) 6364
monocyte chemotaxis
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
neutrophil chemotaxis
calcium-mediated signaling using intracellular calcium source
wound healing
defense response to bacterium
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of interleukin-1 alpha biosynthetic process
positive regulation of nitric-oxide synthase biosynthetic process
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
T cell migration
thymocyte migration
positive regulation of T cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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