Invitrogen

CCL27 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated. View Details

View all (10) CCL27 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

CCL27 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of CCL27/CTACK was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47908) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue).

CCL27 Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouse CCL27/CTACK chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Alamar blue staining. Chemotaxis elicited by recombinant mouse CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47908). The ND50 is typically 10-20 µg/mL.

CCL27 Antibody in Neutralization — Neutralization of CCL27 by (Product # PA5-47908) showing the decrease in signal with increasing concentrations of CCL27 Antibody. Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. {Neu}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

Neutralization (Neu)

10-20 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human CCL27/CTACK Leu26-Asn120

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2576248

Product Specific Information

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CCL27 gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: ALP; C-C motif chemokine 27; CC chemokine ILC; chemokine (C-C motif) ligand 27; CTACK; Cutaneous T-cell-attracting chemokine; ESkine; IL-11 R-alpha-locus chemokine; Skinkine; skinskine; small inducible cytokine A27; small inducible cytokine A27a; Small-inducible cytokine A27

Gene Aliases: ALP; AW558992; Ccl27; CTACK; CTAK; ESkine; ILC; PESKY; Scya27; Scya27a

UniProt ID: (Mouse) Q9Z1X0

Entrez Gene ID: (Mouse) 20301

Molecular Function: chemokine cytokine signaling molecule