Invitrogen

CCL27 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

View all (10) CCL27 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CCL27 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of CCL27/CTACK was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47908) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue).

CCL27 Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouse CCL27/CTACK chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Alamar blue staining. Chemotaxis elicited by recombinant mouse CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47908). The ND50 is typically 10-20 µg/mL.

Neutralization of CCL27 by (Product # PA5-47908) showing the decrease in signal with increasing concentrations of CCL27 Antibody. Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. {Neu}

Product Details

PA5-47908

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	-
Immunohistochemistry (Frozen) (IHC (F))	5-15 µg/mL	-
Neutralization (Neu)	10-20 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived recombinant human CCL27/CTACK Leu26-Asn120
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2576248

Product Specific Information

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CCL27 gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ALP; C-C motif chemokine 27; CC chemokine ILC; chemokine (C-C motif) ligand 27; CTACK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESkine; IL-11 R-alpha-locus chemokine; IL-11 Ralpha-locus chemokine; Skinkine; skinskine; small inducible cytokine A27; small inducible cytokine A27a; small inducible cytokine subfamily A (Cys-Cys), member 27; Small-inducible cytokine A27

Gene Aliases: ALP; AW558992; CCL27; CTACK; CTAK; ESKINE; ILC; PESKY; SCYA27; Scya27a

UniProt ID: (Human) Q9Y4X3, (Mouse) Q9Z1X0

Entrez Gene ID: (Human) 10850, (Mouse) 20301

Molecular Function: chemokine