|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant mouse macrophage inflammatory protein-1alpha, expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.25 µg/ml|
|Inhibition Assays (IA)||3 ug/ml|
|Neutralization (Neu)||3 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24657 detects Macrophage Inflammatory Protein 1 alpha from mouse samples.
PA1-24657 has been successfully used in neutralization, inhibition assays, ELISA, and Western blot procedures.
Mouse MIP1 alpha was tested for its ability to neutralize the bioactivity of recombinant, mouse MIP1 alpha in a cell proliferation assay using hematopoietic stem cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, mouse MIP1 alpha, which is present at five times its own EC50 (the concentration of recombinant, mouse MIP1 alpha producing a one-half maximal bioactivity without antibody).
The PA1-24657 immunogen is recombinant mouse macrophage inflammatory protein-1alpha, expressed in E. coli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.