Invitrogen

CCL3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated. View Details

View all (28) CCL3 (MIP-1 alpha) antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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CCL3 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of recombinant Mouse CCL3/MIP-1αchemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1α (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP-1α Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46951). The ND50 is typically 0.05-0.2 µg/mL.

CCL3 Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouseCCL3/MIP-1a chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-mouse CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46951). The ND50 is typically0.05-0.2 µg/mL.

CCL3 Antibody in Neutralization — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of mouse CCL3 is shown by decrease in RFU (relative fluorescent units - measure of chemotaxis) with increasing concentrations of CCL3 polyclonal antibody (PA5-46951). {Neu}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

Neutralization (Neu)

0.05-0.2 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2609518

Product Specific Information

In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human (rh) CCL3, recombinant mouse (rm) CCL9/10, and rmCCL4 and less than 0.4% cross-reactivity with rhCCL7.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-C motif chemokine 3; H-MIP-1-alpha; Heparin-binding chemotaxis protein; L2G25B; LD78-alpha; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1 alpha; MIP-1-alpha; MIP1 (a); mip1 alpha; RP23-320E6.7; SIS-alpha; small inducible cytokine A3; Small-inducible cytokine A3; TY-5

Gene Aliases: AI323804; Ccl3; G0S19-1; LD78alpha; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; Scya3

UniProt ID: (Mouse) P10855

Entrez Gene ID: (Mouse) 20302

Molecular Function: chemokine cytokine signaling molecule