Invitrogen

CCL3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

View all (27) CCL3 (MIP-1 alpha) antibodies

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Protocols

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CCL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CCL3/MIP-1 alpha was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-human CCL3/MIP-1 alpha Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47000) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes.

CCL3 Antibody in Neutralization (Neu) — Neutralization assay of recombinant human CCL3/MIP-1a chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant humanCCL3/MIP-1a (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-human CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47000). The ND50 is typically 2-8 µg/mL.

CCL3 Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CCL3 is shown by the decrease in mean RFU (relative fluorescent units - measured by Resazurin - measure of chemotaxis) with increasing concentrations of CCL3 Polyclonal Antibody (PA5-47000). {Neu}

Product Details

PA5-47000

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	5-15 µg/mL	-
ELISA (ELISA)	0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)	-
Neutralization (Neu)	2-8 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2609538

Product Specific Information

In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta, rhMIP-1 gamma, rhMIP-3 alpha, rhMIP-3 beta, and recombinant mouse (rm) MIP-1 alpha is observed. This antibody neutralizes the biological activity of recombinant human CCL3/MIP-1 alpha. In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. This antibody will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; LD78-alpha(4-69); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; MIP-1-alpha(4-69); mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein

Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3

UniProt ID: (Human) P10147

Entrez Gene ID: (Human) 6348

Molecular Function: cytokine intercellular signal molecule