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Invitrogen
CCL3 Polyclonal Antibody
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FIGURE: 1 / 3
CCL3 Antibody (PA5-47000) in IHC (P)
Immunohistochemical analysis of CCL3 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated in CCL3 polyclonal antibody (Product # PA5-47000) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes.
Product Details
PA5-47000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta, rhMIP-1 gamma, rhMIP-3 alpha, rhMIP-3 beta, and recombinant mouse (rm) MIP-1 alpha is observed. This antibody neutralizes the biological activity of recombinant human CCL3/MIP-1 alpha. In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. This antibody will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; G0S19-1 peptide precursor; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Gene Aliases: CCL3; G0S19-1; LD78; LD78ALPHA; MIP-1-alpha; MIP1A; SCI; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
MAPK cascade
osteoblast differentiation
cell activation
monocyte chemotaxis
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
cell-cell signaling
regulation of cell shape
response to toxic substance
positive regulation of gene expression
negative regulation of gene expression
T cell chemotaxis
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
signaling
positive regulation of cell migration
negative regulation of bone mineralization
neutrophil chemotaxis
positive regulation of interleukin-1 beta production
positive regulation of tumor necrosis factor production
eosinophil degranulation
positive regulation of neuron apoptotic process
astrocyte cell migration
negative regulation by host of viral transcription
negative regulation of osteoclast differentiation
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
regulation of behavior
positive regulation of calcium-mediated signaling
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
regulation of sensory perception of pain
cell chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
response to cholesterol
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
granulocyte chemotaxis
positive regulation of calcium ion import
positive regulation of microglial cell activation
positive regulation of microglial cell migration
positive regulation of natural killer cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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