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CCL3L1 Antibody (500-P187GBT-500UG) in WB
Western Blot: To detect Human LD78beta by Western Blot analysis CCL3L1 Polyclonal Antibody, Biotin (Product # 500-P187GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human LD78beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P187GBT-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human LD78beta (CCL3L1)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
AA Sequence of recombinant protein: APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human LD78beta (CCL3L1). Anti-Human LD78beta (CCL3L1)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human LD78beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human LD78beta (500-P187G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LD78beta.
Western Blot: To detect Human LD78beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human LD78beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P187GBT-1MG will be provided as 2 x 500 µg
Target Information
This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility. This record represents one of two copies that are present on the ALT_REF_LOCI_2 alternate haplotype of the GRCh38 human reference genome assembly. Alternative splicing of this gene results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 3-like 1; chemokine (C-C motif) ligand 3-like 1; G0/G1 switch regulatory protein 19-2; LD78-beta(1-70); PAT 464.2; small inducible cytokine A3-like 1; Small-inducible cytokine A3-like 1; Tonsillar lymphocyte LD78 beta protein
Gene Aliases: 464.2; CCL3L1; CCL3L3; D17S1718; G0S19-2; LD78; LD78-beta(1-70); LD78BETA; MIP1AP; SCYA3L; SCYA3L1
UniProt ID: (Human) P16619
Entrez Gene ID: (Human) 6349
monocyte chemotaxis
inflammatory response
G-protein coupled receptor signaling pathway
negative regulation of cell proliferation
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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