In direct ELISAs, approximately 10% cross-reactivity with recombinant human CCL4/MIP-1 beta and recombinant mouse CCL4/MIP-1 beta is observed and less than 1% cross-reactivity with recombinant viral CCL4/MIP-1 beta is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
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Protein Aliases: ACT2; AT744.1; C-C motif chemokine 4; C-C motif ligand 4; CC chemokine ligand 4; G-26; LAG1; lymphocyte-activation gene 1; macrophage inflammatory protein 1-beta; MIP1-beta; MIP1B; MIP1B1; SCYA2; SCYA4; Small-inducible cytokine A4
Gene Aliases: CCL4; SCYA4
UniProt ID: (Dog) Q68AZ0
Entrez Gene ID: (Dog) 448786