P230E targets RANTES in ELISA, and WB applications and shows reactivity with Human samples.
The P230E immunogen is recombinant human RANTES.
P230E detects RANTES which has a predicted molecular weight of approximately 8 kDa.
The P230E Rantes polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M230B (biotinylated conjugate of clone VL-1) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody P230E and biotinylated antibody M230B (clone VL-1) have successfully been used in combination with recombinant Rantes protein SRNTS in ELISA applications.
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; RANTES(3-68); RANTES(4-68); regulated upon activation, normally T-expressed, and presumably secreted; SIS ¿¿; SIS-delta; SIS¿¿; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228
Gene Aliases: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228
UniProt ID: (Human) P13501
Entrez Gene ID: (Human) 6352