Invitrogen

CCL5 (RANTES) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

View all (24) CCL5 (RANTES) antibodies

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Datasheet

Protocols

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CCL5 (RANTES) Antibody in Neutralization (Neu) — Neutralization assay of recombinant Feline CCL5/RANTES chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant Feline CCL5/RANTES (80 ng/mL) is neutral-ized (green line) by increasing concen-trations of Sheep Anti-Feline CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47536). The ND50 is typically 0.5-2.5 µg/mL.

CCL5 (RANTES) Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CCL5 is shown by the decrease in chemotaxis in CCR5 transfected BaF3 cells with increasing concentrations of CCL5 Antibody (Product # PA5-47536). {Neu}

Product Details

PA5-47536

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	-
ELISA (ELISA)	0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)	-
Neutralization (Neu)	0.5-2.5 µg/mL	-

Product Specifications
Species Reactivity	Cat
Host/Isotype	Sheep / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived recombinant feline CCL5/RANTES Tyr26-Asn92 (Lys84Tyr and Gly85 del) View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2606493

Product Specific Information

In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human RANTES, recombinant mouse RANTES, and recombinant cotton rat RANTES is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-C motif chemokine 5; MGC17164; OTTHUMP00000197106; SIS δ; SISδ; small inducible cytokine A5; Small-inducible cytokine A5; T-cell-specific protein RANTES

Gene Aliases: CCL5; RANTES; SCYA5

UniProt ID: (Cat) Q8SQ40

Entrez Gene ID: (Cat) 493689