Invitrogen

CCL5 (RANTES) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated. View Details

View all (23) CCL5 (RANTES) antibodies

Datasheet

Datasheet

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CCL5 (RANTES) Antibody in Neutralization (Neu) — Neutralization assay of recombinant Feline CCL5/RANTES chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant Feline CCL5/RANTES (80 ng/mL) is neutral-ized (green line) by increasing concen-trations of Sheep Anti-Feline CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47536). The ND50 is typically 0.5-2.5 µg/mL.

CCL5 (RANTES) Antibody in Neutralization — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CCL5 is shown by the decrease in chemotaxis in CCR5 transfected BaF3 cells with increasing concentrations of CCL5 Antibody (Product # PA5-47536). {Neu}

Product Details

Tested Applications

Dilution

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

Neutralization (Neu)

0.5-2.5 µg/mL

Western Blot (WB)

0.1 µg/mL

Product Specifications

Species Reactivity

Cat

Host / Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant feline CCL5/RANTES Tyr26-Asn92 (Lys84Tyr and Gly85 del)

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2606493

Product Specific Information

In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human RANTES, recombinant mouse RANTES, and recombinant cotton rat RANTES is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; D17S136E; eoCP; eosinophil chemotactic cytokine; MGC17164; SCYA5; SISd; small inducible cytokine A5; Small-inducible cytokine A5; t cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES

Gene Aliases: CCL5; RANTES; SCYA5

UniProt ID: (Cat) Q8SQ40

Entrez Gene ID: (Cat) 493689

Molecular Function: chemokine cytokine signaling molecule

CCL5 (RANTES) Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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