Invitrogen

CCL5 (RANTES) Recombinant Polyclonal Antibody (25HCLC)

Product Image Gallery

CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CCL5/RANTES was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CCL5/RANTES (25HCLC) Recombinant Rabbit Polyclonal Antibody (Product # 710001) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

CCL5 (RANTES) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CCL5/RANTES showing staining in the cytoplasm of paraffin-embedded mouse lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CCL5/RANTES Recombinant Rabbit Polyclonal Antibody (Product # 710001) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

CCL5 (RANTES) Antibody in Western Blot (WB) — Western blot analysis of recombinant mouse RANTES (5 ng, lane1) and recombinant rat Rantes (5 ng, lane 2) using a RANTES Recombinant Rabbit Polyclonal Antibody (Product # 710001) at a dilution of 0.1 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).

Product Details

710001

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-1 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Recombinant Polyclonal
Type	Antibody
Clone	25HCLC
Immunogen	proprietary
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2532515

Product Specific Information

This antibody is predicted to react with rat and human based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dysregulated oxalate metabolism is a driver and therapeutic target in atherosclerosis.

Cell reports

Liu Y,Zhao Y,Shukha Y,Lu H,Wang L,Liu Z,Liu C,Zhao Y,Wang H,Zhao G,Liang W,Fan Y,Chang L,Yurdagul A,Pattillo CB,Orr AW,Aviram M,Wen B,Garcia-Barrio MT,Zhang J,Liu W,Sun D,Hayek T,Chen YE,Rom O

710001 was used in Immunohistochemistry to uncover dysregulated oxalate metabolism via suppressed AGXT as a driver and therapeutic target in atherosclerosis.

Tue Jul 27 00:00:00 EDT 2021

PARP inhibitors promote stromal fibroblast activation by enhancing CCL5 autocrine signaling in ovarian cancer.

NPJ precision oncology

Li X,Fang T,Xu S,Jin P,Zhou D,Wang Z,Li H,Yang Z,Chen G,Zheng X,Xia Y,Wei X,Zhang Z,Yang X,Wang Y,Gao Q

710001 was used in Immunohistochemistry (Paraffin) to to evaluate how cancer-associated fibroblasts react to poly (ADP-ribose) polymerase inhibitors (PARPis) and their potential influence on PARPi resistance in ovarian cancer.

Wed Jun 09 00:00:00 EDT 2021

DSE regulates the malignant characters of hepatocellular carcinoma cells by modulating CCL5/CCR1 axis.

American journal of cancer research

Liao WC,Yen HR,Liao CK,Tseng TJ,Lan CT,Liu CH

710001 was used in Western Blotting to study the role of dermatan sulfate epimerase as a critical mediator of malignant character in hepatocellular carcinoma.

Tue Sep 29 00:00:00 EDT 2020

NADPH Oxidase 4 Regulates Inflammation in Ischemic Heart Failure: Role of Soluble Epoxide Hydrolase.

Antioxidants & redox signaling

Stevenson MD,Canugovi C,Vendrov AE,Hayami T,Bowles DE,Krause KH,Madamanchi NR,Runge MS

710001 was used in Western Blotting to examine the function of NOX4 in the pathogenesis of ischemic heart failure, utilizing mouse models, cell culture, and human heart samples.

Mon Jul 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

Gene Aliases: CCL5; D17S136E; eoCP; MuRantes; RANTES; SCYA5; SIS-delta; SISd; TCP228

UniProt ID: (Human) P13501, (Mouse) P30882

Entrez Gene ID: (Human) 6352, (Rat) 81780, (Mouse) 20304

Molecular Function: cytokine intercellular signal molecule