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CCM3/PDCD10 Polyclonal Antibody

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CCM3/PDCD10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 40x lens).

CCM3/PDCD10 Antibody in Immunohistochemistry (IHC) — WB and IF result of CCM3 antibody (courtesy of Dr. Shah, University of Muenster). IF note: E15 mouse brain cortex was PFA fixed, the sections were antigen retrieved with Na-citrate buffer (pH 6) and blocked with NGS and Triton X100. 1:150 dilution of CCM3 antibody was used with overnight incubation of sections at 4 degrees. Secondary anti-Rabbit conjugated to AF488 was used to detect the staining.

CCM3/PDCD10 Antibody in Western Blot (WB) — Rat skin tissue were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

CCM3/PDCD10 Antibody in Immunoprecipitation (IP) — IP result of anti-CCM3/PDCD10 (IP:10294-2-AP, 3ug; Detection:10294-2-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.

Product Details

10294-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CCM3/PDCD10 Fusion Protein Ag0348 (9-101 aa encoded by BC002506) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.32 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.

Immunogen sequence: KNEAETTSM VSMPLYAVMY PVFNELERVN LSAAQTLRAA FIKAEKENPG LTQDIIMKIL EKKSVEVNFT ESLLRMAADD VEEYMIERPE PEFQ (9-101 aa encoded by BC002506)

Target Information

This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion.

Human mutation

Nardella G,Visci G,Guarnieri V,Castellana S,Biagini T,Bisceglia L,Palumbo O,Trivisano M,Vaira C,Scerrati M,Debrasi D,D'Angelo V,Carella M,Merla G,Mazza T,Castori M,D'Agruma L,Fusco C

Sat Dec 01 00:00:00 EST 2018

Arsenic-induced anti-angiogenesis via miR-425-5p-regulated CCM3.

Toxicology letters

Gao Y,Yin Y,Xing X,Zhao Z,Lu Y,Sun Y,Zhuang Z,Wang M,Ji W,He Y

Fri Jul 08 00:00:00 EDT 2016

Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration.

Development (Cambridge, England)

Louvi A,Nishimura S,Günel M

Sat Mar 01 00:00:00 EST 2014

Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress.

The Journal of biological chemistry

Fidalgo M,Guerrero A,Fraile M,Iglesias C,Pombo CM,Zalvide J

Fri Mar 30 00:00:00 EDT 2012

A novel non-canonical mechanism of regulation of MST3 (mammalian Sterile20-related kinase 3).

The Biochemical journal

Fuller SJ,McGuffin LJ,Marshall AK,Giraldo A,Pikkarainen S,Clerk A,Sugden PH

Thu Mar 15 00:00:00 EDT 2012

Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.

The Journal of clinical investigation

Chan AC,Drakos SG,Ruiz OE,Smith AC,Gibson CC,Ling J,Passi SF,Stratman AN,Sacharidou A,Revelo MP,Grossmann AH,Diakos NA,Davis GE,Metzstein MM,Whitehead KJ,Li DY

Sun May 01 00:00:00 EDT 2011

Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.

The Journal of clinical investigation

Chan AC,Drakos SG,Ruiz OE,Smith AC,Gibson CC,Ling J,Passi SF,Stratman AN,Sacharidou A,Revelo MP,Grossmann AH,Diakos NA,Davis GE,Metzstein MM,Whitehead KJ,Li DY

Sun May 01 00:00:00 EDT 2011

PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival.

The Journal of biological chemistry

Lin C,Meng S,Zhu T,Wang X

Fri Dec 31 00:00:00 EST 2010

PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival.

The Journal of biological chemistry

Lin C,Meng S,Zhu T,Wang X

Fri Dec 31 00:00:00 EST 2010

CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.

Journal of cell science

Fidalgo M,Fraile M,Pires A,Force T,Pombo C,Zalvide J

Thu Apr 15 00:00:00 EDT 2010

CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.

Journal of cell science

Fidalgo M,Fraile M,Pires A,Force T,Pombo C,Zalvide J

Thu Apr 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: apoptosis-related protein 15; Cerebral cavernous malformations 3 protein; MGC1212; MGC24477; PDCD 10; Programmed cell death protein 10; TF-1 cell apoptosis-related protein 15

Gene Aliases: 2410003B13Rik; CCM3; PDCD10; Tfa15; TFAR15

UniProt ID: (Human) Q9BUL8, (Rat) Q6NX65, (Mouse) Q8VE70

Entrez Gene ID: (Human) 11235, (Rat) 494345, (Mouse) 56426

Function(s)

Process(es)

protein binding protein kinase binding protein homodimerization activity protein N-terminus binding

CCM3/PDCD10 Polyclonal Antibody

Product Details

10294-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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