|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues S(18) H S L F T R S I Q E L D E G A T T P Y D Y D D G E P C(45) of mouse CCR2|
|Storage buffer||0.01M potassium phosphate, pH 7.2, with 0.14M NaCl, 1mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:40|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-27409 detects CCR2 from mouse samples.
PA1-27409 has been successfully used in immunohistochemistry (paraffin), immunocytochemistry, flow cytometry, and Western blot procedures.
The PA1-27409 immunogen is a synthetic peptide corresponding to the N-terminal residues S(18) H S L F T R S I Q E L D E G A T T P Y D Y D D G E P C(45) of mouse CCR2
CCR2, or C-C chemokine receptor type 2, is a receptor for several monocyte chemo-attractant proteins (MCP1, MCP3, MCP4) which specifically mediate monocyte chemotaxis. CCR2 transduces such signals by increasing the intracellular level of clcium ions. For example, MCP1 is involved in monocyte infiltration in inflammatory diseases such as rheumatiod arthritis as well as in the inflammatory response against tumors. CCR2 is also an alternative coreceptor with CD4 for HIV-1 infection. CCR2 has two isoforms and has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen and thymus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS.
PA1-27409 was used in immunohistochemistry to study the roles of MCP-1 and CCR2 in astrocytosis in a murine model of ALS
|Kawaguchi-Niida M,Yamamoto T,Kato Y,Inose Y,Shibata N||Acta neuropathologica communications (1:null)||2013|