Invitrogen

CD105 Polyclonal Antibody

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CD105 Antibody in Immunofluorescence (IF) — Immunofluorescence staining of CD105 in U251MG cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD105 Polyclonal Antibody (Product # PA5-80582) (dilution ratio 1:1000) at 4° C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human CD105 in human liver with CD105 Polyclonal Antibody (Product # PA5-80582) (1:5000, formalin-fixed paraffin embedded sections).

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human CD105 in human renal carcinoma with CD105 Polyclonal Antibody (Product # PA5-80582) (1:5000, formalin-fixed paraffin embedded sections).

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human CD105 in human tonsil with CD105 Polyclonal Antibody (Product # PA5-80582) (1:5000, formalin-fixed paraffin embedded sections).

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:5000-1:10000

Immunocytochemistry (ICC)

1:300-1:10000

Immunofluorescence (IF)

1:300-1:10000

Immunohistochemistry (Paraffin) (IHC (P))

1:2000-1:10000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Endoglin/CD105 Protein

Conjugate

Unconjugated

Form

Liquid

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Target Information

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD105; CD105 antigen; END; Endoglin; ENG; HHT1; ORW; ORW1

Gene Aliases: END; ENG; HHT1; ORW1

UniProt ID: (Human) P17813

Entrez Gene ID: (Human) 2022

Molecular Function: TGF-beta receptor cytokine receptor receptor

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation

Process(es)

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding

Suggested Secondary Antibodies