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Invitrogen
Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.
Applications Reported: The OX-86 antibody has been reported for use in flow cytometric analysis. It does not block binding of CD134 to its ligand, but has been reported for use in functional assays.
Applications Tested: The OX-86 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD134; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; Tumor necrosis factor receptor superfamily member 4
Gene Aliases: ACT35; CD134; Ly-70; Ox40; Tnfrsf4; Txgp1; TXGP1L
UniProt ID: (Mouse) P47741
Entrez Gene ID: (Mouse) 22163
Molecular Function:
cytokine receptor
receptor
tumor necrosis factor receptor
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