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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Analyte Specific Reagent
Myocardial ischemia and reperfusion leads to transient CD8 immune deficiency and accelerated immunosenescence in CMV-seropositive patients.
MHCD1428 was used in flow cytometry to study the incidence of coronary heart disease in cytomegalovirus-seropositive individuals.
|Hoffmann J,Shmeleva EV,Boag SE,Fiser K,Bagnall A,Murali S,Dimmick I,Pircher H,Martin-Ruiz C,Egred M,Keavney B,von Zglinicki T,Das R,Todryk S,Spyridopoulos I||Circulation research (116:87)||2015|
Quantitative and qualitative differences in the T cell response to HIV in uninfected Ugandans exposed or unexposed to HIV-infected partners.
MHCD1428 was used in flow cytometry to characterize T cell responses of HIV-exposed, yet persistently uninfected individuals.
|Pala P,Serwanga J,Watera C,Ritchie AJ,Moodie Z,Wang M,Goonetilleke N,Birabwa E,Hughes P,Senkaali D,Nakiboneka R,Grosskurth H,Haynes B,McMichael A,Kaleebu P||Journal of virology (87:9053)||2013|
A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.
MHCD1428 was used in flow cytometry to develop and validate a novel system for high throughput quantitation of autophagy in primary human leukocytes.
|Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK||Autophagy (8:677)||2012|
Atorvastatin induces T cell proliferation by a telomerase reverse transcriptase (TERT) mediated mechanism.
MHCD1428 was used in flow cytometry and immunocytochemistry to study the effect of atorvastatin on telomerase activity in peripheral blood mononuclear cells and T-lymphocytes
|Bennaceur K,Atwill M,Al Zhrany N,Hoffmann J,Keavney B,Breault D,Richardson G,von Zglinicki T,Saretzki G,Spyridopoulos I||Atherosclerosis (236:312)||2014|
CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein