|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Analyte Specific Reagent
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
MHCD1430 was used in flow cytometry to study the dynamics of Ag-specific CD4(+) T cells during antiretroviral therapy.
|Riou C,Tanko RF,Soares AP,Masson L,Werner L,Garrett NJ,Samsunder N,Abdool Karim Q,Abdool Karim SS,Burgers WA||Journal of immunology (Baltimore, Md. : 1950) (195:2273)||2015|
Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry.
MHCD1430 was used in flow cytometry to describe protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells in peripheral blood
|Fung E,Esposito L,Todd JA,Wicker LS||Nature protocols (5:357)||2010|