Invitrogen

CD16 Monoclonal Antibody (3G8), APC

Product Details

MHCD1605

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	Assay-Dependent	View 7 publications 7 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	3G8
Immunogen	Human CD16
Conjugate	APC Alexa Fluor 700 FITC Pacific Blue Pacific Orange PE PE-Alexa Fluor 700 PE-Texas Red PerCP
Excitation/Emission Profile	View spectra
Form	Liquid
Purification	purified
Storage buffer	PBS with sucrose, BSA
Contains	0.1% sodium azide
Storage conditions	4° C, store in dark
RRID	AB_10371750

Product Specific Information

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

Target Information

CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

Analyte Specific Reagent

Flow Cytometry

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Product References

A comprehensive characterization of aggravated aging-related changes in T lymphocytes and monocytes in end-stage renal disease: the iESRD study.

Immunity & ageing : I & A

Chiu YL,Shu KH,Yang FJ,Chou TY,Chen PM,Lay FY,Pan SY,Lin CJ,Litjens NHR,Betjes MGH,Bermudez S,Kao KC,Chia JS,Wang G,Peng YS,Chuang YF

MHCD1605 was used in Flow cytometry/Cell sorting to demonstrates the potential link between immunosenescence in end-stage renal disease and duration of exposure to the uremic milieu.

Sat Apr 09 00:00:00 EDT 2022

Effects of acute exercise on monocyte subpopulations in metabolic syndrome patients.

Cytometry. Part B, Clinical cytometry

Wonner R,Wallner S,Orsó E,Schmitz G

MHCD1605 was used in flow cytometry to report the exercise-induced changes on the blood cell composition of patients with metabolic syndrome

Sun Jul 01 00:00:00 EDT 2018

Cerebral ischemia initiates an immediate innate immune response in neonates during cardiac surgery.

Journal of neuroinflammation

Algra SO,Groeneveld KM,Schadenberg AW,Haas F,Evens FC,Meerding J,Koenderman L,Jansen NJ,Prakken BJ

MHCD1605 was used in flow cytometry to study the immediate innate immune response after an ischemic insult.

Thu Feb 07 00:00:00 EST 2013

Variations in the heme oxygenase-1 microsatellite polymorphism are associated with plasma CD14 and viral load in HIV-infected African-Americans.

Genes and immunity

Seu L,Burt TD,Witte JS,Martin JN,Deeks SG,McCune JM

MHCD1605 was used in flow cytometry to study heme oxygenase- in HIV-infected people.

Sun Apr 01 00:00:00 EDT 2012

Specific expression of GPR56 by human cytotoxic lymphocytes.

Journal of leukocyte biology

Peng YM,van de Garde MD,Cheng KF,Baars PA,Remmerswaal EB,van Lier RA,Mackay CR,Lin HH,Hamann J

MHCD1605 was used in Flow cytometry/Cell sorting to report the existence of a new cluster of adhesion-GPCRs in human immune cells.

Sat Oct 01 00:00:00 EDT 2011

Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus.

Allergy

Bratke K,Klein C,Kuepper M,Lommatzsch M,Virchow JC

MHCD1605 was used in flow cytometry to test if Th1- and Th2-like conditions alter antigen expression and function of plasmacytoid dendritic cells

Tue Mar 01 00:00:00 EST 2011

Regulation of Toll-like receptor (TLR)2 and TLR4 on CD14dimCD16+ monocytes in response to sepsis-related antigens.

Clinical and experimental immunology

Skinner NA,MacIsaac CM,Hamilton JA,Visvanathan K

MHCD1605 was used in Flow cytometry/Cell sorting to hypothesize that following exposure to bacterial antigens, subsequent TNF-alpha drives a differentiation of monocytes into a CD14(dim)CD16(+) subpopulation.

Mon Aug 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

UniProt ID: (Human) P08637, (Human) O75015

Entrez Gene ID: (Human) 2214, (Human) 2215

Molecular Function: immunoglobulin receptor superfamily