|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Analyte Specific Reagent
Utilility of flow cytometry as ancillary study to improve the cytologic diagnosis of thyroid lymphomas.
MHCD1630 was used in flow cytometry to assess the efficacy of using flow cytometry immunophenotyping with fine-needle aspiration cytology for the diagnosis of thyroid lymphoma.
|Stacchini A,Pacchioni D,Demurtas A,Aliberti S,Cassenti A,Isolato G,Gazzera C,Veltri A,Sapino A,Papotti M,Freddi M,Palestini N,Sisto G,Novero D||Cytometry. Part B, Clinical cytometry (88:320)||2015|
LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells.
MHCD1630 was used in flow cytometry to test if leukocyte Ig-like receptor A2 regulates DC differentiation using a leprosy model.
|Lee DJ,Sieling PA,Ochoa MT,Krutzik SR,Guo B,Hernandez M,Rea TH,Cheng G,Colonna M,Modlin RL||Journal of immunology (Baltimore, Md. : 1950) (179:8128)||2007|
Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.
MHCD1630 was used in flow cytometry to characterize B7-H3.
|Steinberger P,Majdic O,Derdak SV,Pfistershammer K,Kirchberger S,Klauser C,Zlabinger G,Pickl WF,Stöckl J,Knapp W||Journal of immunology (Baltimore, Md. : 1950) (172:2352)||2004|
Prospective isolation of human clonogenic common myeloid progenitors.
MHCD1630 was used in flow cytometry to report how to isolate highly purified hematopoietic intermediates.
|Manz MG,Miyamoto T,Akashi K,Weissman IL||Proceedings of the National Academy of Sciences of the United States of America (99:11872)||2002|
Prognostic significance of CD56 antigen expression in acute myeloid leukemia.
MHCD1630 was used in flow cytometry to examine CD56 expression in a cohort of acute myeloid leukemia patients in to assess its frequency and prognostic relevance.
|Di Bona E,Sartori R,Zambello R,Guercini N,Madeo D,Rodeghiero F||Haematologica (87:250)||2002|
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
MHCD1630 was used in flow cytometry to characterize the localization and function of STRL33/Bonzo.
|Sharron M,Pöhlmann S,Price K,Lolis E,Tsang M,Kirchhoff F,Doms RW,Lee B||Blood (96:41)||2000|
Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.
MHCD1630 was used in flow cytometry to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites on various T cells and macrophages.
|Lee B,Sharron M,Montaner LJ,Weissman D,Doms RW||Proceedings of the National Academy of Sciences of the United States of America (96:5215)||1999|
Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions.
MHCD1630 was used in flow cytometry and immunoprecipitation to compare Fc gamma RIII(CD16) transcripts isolated from PMN and NK cells.
|Ravetch JV,Perussia B||The Journal of experimental medicine (170:481)||1989|
Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.
MHCD1630 was used in immunoprecipitation to identify a locus for at least two Fc gamma R genes on human chromosome 1.
|Peltz GA,Grundy HO,Lebo RV,Yssel H,Barsh GS,Moore KW||Proceedings of the National Academy of Sciences of the United States of America (86:1013)||1989|
B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.
MHCD1630 was used in ELISA to identify immunoregulatory molecules on dendritic cells.
|Selenko-Gebauer N,Majdic O,Szekeres A,Höfler G,Guthann E,Korthäuer U,Zlabinger G,Steinberger P,Pickl WF,Stockinger H,Knapp W,Stöckl J||Journal of immunology (Baltimore, Md. : 1950) (170:3637)||2003|