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CD164 Antibody (PA5-104047) in ICC/IF
Immunocytochemistry analysis of CD164 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CD164 polyclonal antibody (Product # PA5-104047) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104047
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CD164(Accession Q04900), corresponding to amino acid residues D99-V149.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853377
Product Specific Information
Antibody detects endogenous levels of total CD164.
Target Information
This gene encodes a transmembrane sialomucin and cell adhesion molecule that regulates the proliferation, adhesion and migration of hematopoietic progenitor cells. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Myc binding protein; CD164; CD164 antigen; CD164 antigen, sialomucin; CD164 molecule, sialomucin; CD164 variant C; deafness, autosomal dominant 66; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; Sialomucin core protein 24
Gene Aliases: A115; A24; AA589639; AW540279; CD164; DFNA66; endolyn; MGC-24; MGC-24v; MSSP; MUC-24
UniProt ID: (Human) Q04900, (Rat) Q9QX82, (Mouse) Q9R0L9
Entrez Gene ID: (Human) 8763, (Rat) 83689, (Mouse) 53599
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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