Invitrogen

CD171 Monoclonal Antibody (eBio5G3 (5G3)), Biotin, eBioscience™

9 Published Figures

23 References

View all (91) CD171 (L1CAM) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

FIGURE: 1 / 10

CD171 Antibody (13-1719-82) in Flow

Staining of Panc-1 cell line with 0.25 µg of Mouse IgG2a kappa Isotype Control Biotin (Product # 13-4724-85) (blue histogram) or 0.25 µg of Anti-Human CD171 Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD171 Antibody in Western Blot, Immunoprecipitation (WB, IP)

CD171 Antibody in Immunohistochemistry (IHC)

CD171 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

13-1719-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

ELISA (ELISA)

View 3 publications 3 publications

Immunoprecipitation (IP)

View 4 publications 4 publications

Affinity Purification (AP)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio5G3 (5G3)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2043813

Product Specific Information

Description: The monoclonal antibody eBio5G3 recognizes CD171 also known as neural cell adhesion molecule L1. CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats. CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines. Expression of CD171 on tumors is thought to contribute to tumor progression. Epitope of eBio5G3 is in amino-terminal Ig-like domain.

Applications Reported: This eBio5G3 (5G3) antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested: This eBio5G3 (5G3) antibody has been tested by flow cytometric analysis of Panc-1 cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint.

RNA biology

Dunlop RA,Banack SA,Cox PA

13-1719-82 was used in Immunocapture to conclude that L1CAM immunocapture generates a unique fraction of EVs that consistently and robustly replicates a miRNA fingerprint which differentiates ALS patients from controls.

Sun Jan 01 00:00:00 EST 2023

Neuronally-enriched exosomal microRNA-27b mediates acute effects of ibuprofen on reward-related brain activity in healthy adults: a randomized, placebo-controlled, double-blind trial.

Scientific reports

Burrows K,Figueroa-Hall LK,Kuplicki R,Stewart JL,Alarbi AM,Ramesh R,Savitz JB,Teague TK,Risbrough VB,Paulus MP

13-1719-82 was used in Western Blot, Flow Cytometry to demonstrate that miR-27b could be an important messaging molecule that is associated with regulating the processing of positive or negative valenced information.

Mon Jan 17 00:00:00 EST 2022

Neuronally-enriched exosomal microRNA-27b mediates acute effects of ibuprofen on reward-related brain activity in healthy adults: a randomized, placebo-controlled, double-blind trial.

Scientific reports

Burrows K,Figueroa-Hall LK,Kuplicki R,Stewart JL,Alarbi AM,Ramesh R,Savitz JB,Teague TK,Risbrough VB,Paulus MP

Mon Jan 17 00:00:00 EST 2022

Evaluation of blood-based, extracellular vesicles as biomarkers for aging-related TDP-43 pathology.

Alzheimer's & dementia (Amsterdam, Netherlands)

Winston CN,Sukreet S,Lynch H,Lee VM,Wilcock DM,Nelson PT,Rissman RA

13-1719-82 was used in Flow cytometry/Cell sorting to evaluate TDP-43 in plasma ADEVs derived from autopsy confirmed LATE-NC subjects, with or without comorbid Alzheimer's disease pathology.

Wed Jun 07 00:00:00 EDT 2023

Evaluation of blood-based, extracellular vesicles as biomarkers for aging-related TDP-43 pathology.

Alzheimer's & dementia (Amsterdam, Netherlands)

Winston CN,Sukreet S,Lynch H,Lee VM,Wilcock DM,Nelson PT,Rissman RA

13-1719-82 was used in Flow cytometry/Cell sorting to evaluate TDP-43 in plasma ADEVs derived from autopsy confirmed LATE-NC subjects, with or without comorbid Alzheimer's disease pathology.

Wed Jun 07 00:00:00 EDT 2023

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Western Blot

Sat Jan 01 00:00:00 EST 2022

Insulin receptor substrate in brain-enriched exosomes in subjects with major depression: on the path of creation of biosignatures of central insulin resistance.

Molecular psychiatry

Nasca C,Dobbin J,Bigio B,Watson K,de Angelis P,Kautz M,Cochran A,Mathé AA,Kocsis JH,Lee FS,Murrough JW,McEwen BS,Rasgon N

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Western Blot

Wed Sep 01 00:00:00 EDT 2021

Insulin receptor substrate in brain-enriched exosomes in subjects with major depression: on the path of creation of biosignatures of central insulin resistance.

Molecular psychiatry

Nasca C,Dobbin J,Bigio B,Watson K,de Angelis P,Kautz M,Cochran A,Mathé AA,Kocsis JH,Lee FS,Murrough JW,McEwen BS,Rasgon N

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Western Blot

Wed Sep 01 00:00:00 EDT 2021

Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations.

Cells

Sun B,Tang N,Peluso MJ,Iyer NS,Torres L,Donatelli JL,Munter SE,Nixon CC,Rutishauser RL,Rodriguez-Barraquer I,Greenhouse B,Kelly JD,Martin JN,Deeks SG,Henrich TJ,Pulliam L

13-1719 was used in Immunopurification to investigate the mechanisms to explain long-term neurological sequelae following COVID-19 recovery.

Sat Feb 13 00:00:00 EST 2021

Neuronal and Astrocytic Extracellular Vesicle Biomarkers in Blood Reflect Brain Pathology in Mouse Models of Alzheimer's Disease.

Cells

Delgado-Peraza F,Nogueras-Ortiz CJ,Volpert O,Liu D,Goetzl EJ,Mattson MP,Greig NH,Eitan E,Kapogiannis D

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Western Blot

Fri Apr 23 00:00:00 EDT 2021

Plasma Levels of Neuron- and Astrocyte-Derived Exosomal Amyloid Beta1-42, Amyloid Beta1-40, and Phosphorylated Tau Levels in Schizophrenia Patients and Non-psychiatric Comparison Subjects: Relationships With Cognitive Functioning and Psychopathology.

Frontiers in psychiatry

Lee EE,Winston-Gray C,Barlow JW,Rissman RA,Jeste DV

13-1719 was used in Flow cytometry/Cell sorting to study exosomal amyloid and tau protein levels in people with schizophrenia.

Sat Mar 27 00:00:00 EDT 2021

Frontiers in psychiatry

Lee EE,Winston-Gray C,Barlow JW,Rissman RA,Jeste DV

13-1719 was used in Flow cytometry/Cell sorting to study exosomal amyloid and tau protein levels in people with schizophrenia.

Sat Mar 27 00:00:00 EDT 2021

An miRNA fingerprint using neural-enriched extracellular vesicles from blood plasma: towards a biomarker for amyotrophic lateral sclerosis/motor neuron disease.

Open biology

Banack SA,Dunlop RA,Cox PA

13-1719 was used in Western Blotting to report eight miRNA sequences which significantly distinguish ALS/MND patients from controls in a replicated experiment using a second cohort of patients and controls.

Mon Jun 01 00:00:00 EDT 2020

Assessing Neuronal and Astrocyte Derived Exosomes From Individuals With Mild Traumatic Brain Injury for Markers of Neurodegeneration and Cytotoxic Activity.

Frontiers in neuroscience

Winston CN,Romero HK,Ellisman M,Nauss S,Julovich DA,Conger T,Hall JR,Campana W,O'Bryant SE,Nievergelt CM,Baker DG,Risbrough VB,Rissman RA

13-1719-82 was used in Flow Cytometry to examine the potential of neuronally-derived (NDE) and astrocytic-derived (ADE) exosome cargo proteins as biomarkers of chronic mTBI in younger adults by examing plasma exosomes from a prospective longitudinal study of combat-related risk and resilience, marine resiliency study II (MRSII).

Thu Oct 01 00:00:00 EDT 2020

Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization.

Nature cell biology

Er EE,Valiente M,Ganesh K,Zou Y,Agrawal S,Hu J,Griscom B,Rosenblum M,Boire A,Brogi E,Giancotti FG,Schachner M,Malladi S,Massagué J

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Immunohistochemistry (PFA fixed)

Wed Aug 01 00:00:00 EDT 2018

Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization.

Nature cell biology

Er EE,Valiente M,Ganesh K,Zou Y,Agrawal S,Hu J,Griscom B,Rosenblum M,Boire A,Brogi E,Giancotti FG,Schachner M,Malladi S,Massagué J

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Immunohistochemistry (PFA fixed)

Wed Aug 01 00:00:00 EDT 2018

Increased DJ-1 and α-Synuclein in Plasma Neural-Derived Exosomes as Potential Markers for Parkinson's Disease.

Frontiers in aging neuroscience

Zhao ZH,Chen ZT,Zhou RL,Zhang X,Ye QY,Wang YZ

13-1719 was used in Immunoprecipitation to show that DJ-1 in plasma neural-derived exosomes may be used as a potential biomarker as α-synuclein in PD and they might participate in the mechanism of PD together.

Thu Oct 01 00:00:00 EDT 2020

Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles.

International journal of molecular sciences

Guix FX,Corbett GT,Cha DJ,Mustapic M,Liu W,Mengel D,Chen Z,Aikawa E,Young-Pearse T,Kapogiannis D,Selkoe DJ,Walsh DM

13-1719-82 was used in Affinity Purification to demonstrate the presence of minute amounts of free-floating and exosome-contained full-length tau in human biofluids.

Tue Feb 27 00:00:00 EST 2018

Exosomal biomarkers of brain insulin resistance associated with regional atrophy in Alzheimer's disease.

Human brain mapping

Mullins RJ,Mustapic M,Goetzl EJ,Kapogiannis D

13-1719 was used in Immunoprecipitation to characterise exosomal biomarkers of brain insulin resistance associated with regional atrophy in Alzheimer's disease.

Sat Apr 01 00:00:00 EDT 2017

Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes.

Frontiers in neuroscience

Mustapic M,Eitan E,Werner JK,Berkowitz ST,Lazaropoulos MP,Tran J,Goetzl EJ,Kapogiannis D

13-1719 was used in Immunoprecipitation to utilise harvested extracellular vesicles enriched for neuronal origin, from peripheral blood as a biomarker discovery platform for neurological disorders.

Mon May 03 00:00:00 EDT 2021

Plasma neuronal exosomal levels of Alzheimer's disease biomarkers in normal aging.

Annals of clinical and translational neurology

Abner EL,Jicha GA,Shaw LM,Trojanowski JQ,Goetzl EJ

13-1719 was used in an ELISA assay to define changes in levels of plasma neuronal exosomal proteins associated with normal ageing in cognitively intact subjects.

Sun May 01 00:00:00 EDT 2016

Prediction of conversion from mild cognitive impairment to dementia with neuronally derived blood exosome protein profile.

Alzheimer's & dementia (Amsterdam, Netherlands)

Winston CN,Goetzl EJ,Akers JC,Carter BS,Rockenstein EM,Galasko D,Masliah E,Rissman RA

13-1719 was used in in vitro experiments to conclude that plasma NDEs from demented patients seeded tau aggregation and induced AD-like neuropathology in normal mouse CNS.

Wed Jul 13 00:00:00 EDT 2016

Altered lysosomal proteins in neural-derived plasma exosomes in preclinical Alzheimer disease.

Neurology

Goetzl EJ,Boxer A,Schwartz JB,Abner EL,Petersen RC,Miller BL,Kapogiannis D

13-1719 was used in an ELISA assay to confirm early neuronal lysosomal dysfunction in Alzheimer disease patients, and suggest that these proteins may be useful biomarkers in large prospective studies.

Tue Jul 07 00:00:00 EDT 2015

Low neural exosomal levels of cellular survival factors in Alzheimer's disease.

Annals of clinical and translational neurology

Goetzl EJ,Boxer A,Schwartz JB,Abner EL,Petersen RC,Miller BL,Carlson OD,Mustapic M,Kapogiannis D

13-1719 was used in an ELISA assay to quantify transcription factors that mediate neuronal defenses against stress in plasma neural-derived exosomes of Alzheimer's disease or frontotemporal dementia patients and matched controls.

Wed Jul 01 00:00:00 EDT 2015

Serpins promote cancer cell survival and vascular co-option in brain metastasis.

Cell

Valiente M,Obenauf AC,Jin X,Chen Q,Zhang XH,Lee DJ,Chaft JE,Kris MG,Huse JT,Brogi E,Massagué J

Published figure using CD171 monoclonal antibody (Product # 13-1719-82) in Western Blot

Thu Feb 27 00:00:00 EST 2014

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

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Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

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UniProt ID: (Human) P32004

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Entrez Gene ID: (Human) 3897

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Process(es)

chemotaxis cell adhesion nervous system development axon guidance positive regulation of axon extension leukocyte migration

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD171 Monoclonal Antibody (eBio5G3 (5G3)), Biotin, eBioscience™

CD171 Antibody (13-1719-82) in Flow

Product Details

13-1719-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Affinity Purification (AP)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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