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Immunohistochemistry was performed on formalin-fixed, paraffin-embedded canine B cell lymphoma using an automated staining platform. Following deparaffinization, antigen retrieval was performed using CC1 retrieval solution for 30 minutes followed by 32 minute primary antibody incubation with rabbit anti-CD20 polyclonal antibody (Product # PA5-16701) at a dilution of 1:100. Detection was performed using an indirect biotin-streptavidin system and a DAB Detection kit. Data courtesy of the Innovators Program.
|Tested species reactivity||Dog, Cat, Human, Mouse|
|Published species reactivity||Dog, Bovine, Mouse, Human, Horse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human CD20 protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400-800|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16701 targets CD20 in IHC (P), FACS and WB applications and shows reactivity with Human, Canine, Feline and Mouse samples.
The PA5-16701 immunogen is synthetic peptide derived from C-terminus of human CD20 protein.
CD20 is a 33kDa leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Canine indolent and aggressive lymphoma: clinical spectrum with histologic correlation.
PA5-16701 was used in immunohistochemistry - paraffin section to assess the effect of chemotherapy on canine indolent and aggressive lymphoma
|Aresu L,Martini V,Rossi F,Vignoli M,Sampaolo M,Aricò A,Laganga P,Pierini A,Frayssinet P,Mantovani R,Marconato L||Veterinary and comparative oncology (13:348)||2015|
Pathology of fatal lineage 1 and 2 West Nile virus infections in horses in South Africa.
PA5-16701 was used in immunohistochemistry to describe the lesions of six South African horses infected with West Nile virus
|Williams JH,van Niekerk S,Human S,van Wilpe E,Venter M||Journal of the South African Veterinary Association (85:null)||2015|
Intraocular and periocular lymphoma in dogs and cats: a retrospective review of 21 cases (2001-2012).
PA5-16701 was used in immunohistochemistry to review multiple cases of cat and dog intraocular and periocular tumors
|Ota-Kuroki J,Ragsdale JM,Bawa B,Wakamatsu N,Kuroki K||Veterinary ophthalmology (17:389)||2014|
Regulation of the development of asthmatic inflammation by in situ CD4(+)Foxp3 (+) T cells in a mouse model of late allergic asthma.
PA5-16701 was used in immunohistochemistry to study the role of CD4(+)/Foxp3(+) T-cells in modulating inflammation in a murine model of late allergic asthma
|Nakashima T,Hayashi T,Mizuno T||Inflammation (37:1642)||2014|
Immunohistochemical expression of cyclooxygenase-2 in normal, hyperplastic and neoplastic canine lymphoid tissues.
PA5-16701 was used in immunohistochemistry to examine normal, hyperplastic and neoplastic dog lymph nodes for the immunohistochemical expression of COX-2
|Asproni P,Vignoli M,Cancedda S,Millanta F,Terragni R,Poli A||Journal of comparative pathology (151:35)||2014|
BLT-humanized C57BL/6 Rag2-/-γc-/-CD47-/- mice are resistant to GVHD and develop B- and T-cell immunity to HIV infection.
PA5-16701 was used in immunohistochemistry to study the suitability of the BLT-humanized C57BL/6 Rag2-/-gamma-c-/-CD47-/- mouse strain for the study of human pathogens
|Lavender KJ,Pang WW,Messer RJ,Duley AK,Race B,Phillips K,Scott D,Peterson KE,Chan CK,Dittmer U,Dudek T,Allen TM,Weissman IL,Hasenkrug KJ||Blood (122:4013)||2013|
Safety and immunomodulatory effects of allogeneic canine adipose-derived mesenchymal stromal cells transplanted into the region of the lacrimal gland, the gland of the third eyelid and the knee joint.
PA5-16701 was used in immunohistochemistry to transplant allogenic canine adipose-derived mesenchymal stem cells into different anatomical sites to study their safety profile and immune responses
|Park SA,Reilly CM,Wood JA,Chung DJ,Carrade DD,Deremer SL,Seraphin RL,Clark KC,Zwingenberger AL,Borjesson DL,Hayashi K,Russell P,Murphy CJ||Cytotherapy (15:1498)||2013|
Digital dermatitis in cattle is associated with an excessive innate immune response triggered by the keratinocytes.
PA5-16701 was used in immunohistochemistry to study the role of keratinocyte-mediated stimulation of the innate immune response in the pathogenesis of digital dermatitis in cattle
|Refaai W,Ducatelle R,Geldhof P,Mihi B,El-shair M,Opsomer G||BMC veterinary research (9:null)||2013|
Spinal meningeal oligodendrogliomatosis in two boxer dogs.
PA5-16701 was used in immunohistochemistry to report on two cases of spinal meningeal oligodendrogliomatosis in boxer dogs
|Kovi RC,Wünschmann A,Armién AG,Hall K,Carlson T,Shivers J,Oglesbee MJ||Veterinary pathology (50:761)||2013|
Clinical characteristics and outcome in dogs with splenic marginal zone lymphoma.
PA5-16701 was used in immunohistochemistry to study the clinicopathology of splenic marginal zone lymphoma in dogs and the effect of splenectomy on survival
|O'Brien D,Moore PF,Vernau W,Peauroi JR,Rebhun RB,Rodriguez CO,Skorupski KA||Journal of veterinary internal medicine / American College of Veterinary Internal Medicine (27:949)||2013|
Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi.
PA5-16701 was used in immunohistochemistry to study the mechanism of vaccine-induced immunity to nematode infection in cattle and the potential involvement of granulysin and granzyme B
|Van Meulder F,Van Coppernolle S,Borloo J,Rinaldi M,Li RW,Chiers K,Van den Broeck W,Vercruysse J,Claerebout E,Geldhof P||Infection and immunity (81:1798)||2013|
The dog as a possible animal model for human non-Hodgkin lymphoma: a review.
PA5-16701 was used in immunohistochemistry to review the suitability of the dog as a non-Hodgkin lymphoma model
|Marconato L,Gelain ME,Comazzi S||Hematological oncology (31:1)||2013|
Canine inflamed nonepitheliotropic cutaneous T-cell lymphoma: a diagnostic conundrum.
PA5-16701 was used in immunohistochemistry to identify suitable diagnostic parameters for canine inflamed nonepitheliotropic cutaneous T-cell lymphoma
|Moore PF,Affolter VK,Keller SM||Veterinary dermatology (24:204)||2013|
Diagnostic yield and adverse effects of MRI-guided free-hand brain biopsies through a mini-burr hole in dogs with encephalitis.
PA5-16701 was used in immunohistochemistry to study the diagnostic value and clinical risk of MRI-guided free-hand brain biopsies in dogs suspected of suffering from encephalitis
|Flegel T,Oevermann A,Oechtering G,Matiasek K||Journal of veterinary internal medicine / American College of Veterinary Internal Medicine (26:969)||2012|
CD20+ tumor-infiltrating lymphocytes have an atypical CD27- memory phenotype and together with CD8+ T cells promote favorable prognosis in ovarian cancer.
PA5-16701 was used in immunohistochemistry to study the role of CD20+ tumor-infiltrating lymphocytes in humoral and cellular tumor immunity in ovarian cancer
|Nielsen JS,Sahota RA,Milne K,Kost SE,Nesslinger NJ,Watson PH,Nelson BH||Clinical cancer research : an official journal of the American Association for Cancer Research (18:3281)||2012|
Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain.
PA5-16701 was used in immunohistochemistry to study the effect of vaccination on macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with Mycoplasma hyopneumoniae
|Vranckx K,Maes D,Marchioro SB,Villarreal I,Chiers K,Pasmans F,Haesebrouck F||BMC veterinary research (8:null)||2012|
Development and characterization of 5 canine B-cell lymphoma cell lines.
PA5-16701 was used in immunohistochemistry to develop and characterize five canine B-cell lymphoma cell lines
|Zwingenberger AL,Vernau W,Shi C,Yan W,Chen X,Gordon IK,Kent MS||Leukemia research (36:601)||2012|
A retrospective study of the clinical, histological, and immunohistochemical manifestations of 5 dogs originally diagnosed histologically as necrotizing scleritis.
PA5-16701 was used in immunohistochemistry to study the clinicopathology of five dogs diagnosed histologically with necrotizing scleritis
|Denk N,Sandmeyer LS,Lim CC,Bauer BS,Grahn BH||Veterinary ophthalmology (15:102)||2012|
Intramuscular inoculation of cattle with Sarcocystis antigen results in focal eosinophilic myositis.
PA5-16701 was used in immunohistochemistry to study the induction of focal eosinophilic myositis by the intramuscular injection of cows with Sarcocystis antigen
|Vangeel L,Houf K,Geldhof P,Nollet H,Vercruysse J,Ducatelle R,Chiers K||Veterinary parasitology (183:224)||2012|
Use of tissue microarray and automated quantitative analysis for screening and validation of potential biomarkers in mantle cell lymphoma.
PA5-16701 was used in immunohistochemistry to evaluate tissue microarray and automated quantitative analysis for the identification of tumor biomarkers
|Yang DT,Quann PJ,Petrich AM,Leith CP,Young KH,Kahl BS||Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry (19:62)||2011|
Hemophagocytic histiocytic sarcoma in a Japanese black cow.
PA5-16701 was used in immunohistochemistry to investigate histiocytic proliferative disorders in a case of 11-year-old Japanese Black cow
|Matsuda K,Nomoto H,Kawamura Y,Someya Y,Koiwa M,Taniyama H||Veterinary pathology (47:339)||2010|
B-cell intestinal lymphoma with Mott cell differentiation in a 1-year-old miniature Dachshund.
PA5-16701 was used in immunohistochemistry to investigate the clinicopathological features of B-cell intestinal lymphoma with Mott cell differentiation in a miniature Dachshund
|Kodama A,Sakai H,Kobayashi K,Mori T,Maruo K,Kudo T,Yanai T,Masegi T||Veterinary clinical pathology / American Society for Veterinary Clinical Pathology (37:409)||2008|
Feline pulmonary Langerhans cell histiocytosis with multiorgan involvement.
PA5-16701 was used in immunohistochemistry to examine the clinicopathologic features of feline pulmonary Langerhans cell histiocytosis
|Busch MD,Reilly CM,Luff JA,Moore PF||Veterinary pathology (45:816)||2008|
Proliferation of immature plasma cells in pouchitis mucosa in patients with ulcerative colitis.
PA5-16701 was used in immunohistochemistry to study the abnormal plasma cell proliferation in pouchitis mucosa from patients with ulcerative colitis
|Hirata N,Oshitani N,Kamata N,Sogawa M,Yamagami H,Watanabe K,Watanabe T,Tominaga K,Fujiwara Y,Maeda K,Hirakawa K,Arakawa T||Inflammatory bowel diseases (14:1084)||2008|
B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20; CD20 antigen; CD20 receptor; LEU-16; leukocyte surface antigen Leu-16; lymphocyte antigen 44; membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969; MS4A1
AA960661; B1; Bp35; CD20; CVID5; LEU-16; Ly-44; MS4A1; MS4A2; S7