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Invitrogen
CD20 Polyclonal Antibody
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FIGURE: 1 / 37
CD20 Antibody (PA5-16701) in ICC/IF
Immunofluorescence analysis of CD20 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD20 Polyclonal Antibody (Product # PA5-16701) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilu... View More
Product Details
PA5-16701
Applications
Tested Dilution
Publications
Product Specifications
Published species
Avian,
Bovine,
C. elegans,
Cat,
Dog,
Horse,
Human,
Mink,
Mouse,
Mustelid,
Non-human primate,
Pig,
Rat,
Rhesus monkey,
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from C-terminus of human CD20 protein
Conjugate
Form
Liquid
Concentration
0.077 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Upon delivery aliquot and store at -20°C, avoiding freeze/thaw cycles.
For IHC staining, antigen retrieval is not essential but may optimize staining. Recommended positive controls: BJAB WCL and tonsil.
Target Information
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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