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          • Primary Antibodies ›
          • CD22 Antibodies

          Invitrogen

          CD22 Monoclonal Antibody (IS7), FITC

          View all (120) CD22 antibodies
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          Cite CD22 Monoclonal Antibody (IS7), FITC

          • Antibody Testing Data (2)
          CD22 Antibody in Flow Cytometry (Flow)
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          CD22 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          CD22 Antibody (MA1-10144) in Flow

          Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (IS7) FITC Monoclonal antibody (Product # MA1-10144) using a dilution of 20 µL/100 µL of peripheral whole blood. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD22 Antibody in Flow Cytometry (Flow)
          CD22 Antibody in Flow Cytometry (Flow)

          Product Details

          MA1-10144

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          20 µL/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          IS7

          Immunogen

          Reh human cell line
          View immunogen

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • APC 
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Purification

          Size-exclusion chromatography

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          15mM sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11154156

          Product Specific Information

          This antibody reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

          Target Information

          CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. CD22 preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 is also strongly expressed in hairy cell leukemia. CD22 is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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          Cite this product

          Bioinformatics

          Protein Aliases: B-cell receptor CD22; B-lymphocyte cell adhesion molecule; B-lymphocyte cell adhesion molecule (BL-CAM); BL-CAM; CD22; CD22 antigen; FLJ22814; Lectin 2; Leu-14; MGC130020; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2 (Siglec-2); Siglec-2; T-cell surface antigen Leu-14

          View more View less

          Gene Aliases: CD22; SIGLEC-2; SIGLEC2

          View more View less

          UniProt ID: (Human) Q32M46

          View more View less

          Entrez Gene ID: (Human) 933

          View more View less

          Function(s)
          protein binding carbohydrate binding
          Process(es)
          cell adhesion
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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