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CD248 Antibody (PA5-88715) in WB
Western blot analysis of CD248 in extracts of various cell lines. Samples were incubated with CD248 Polyclonal antibody (Product # PA5-88715) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88715
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-150 of human CD248 (NP_0651371)
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805086
Product Specific Information
Immunogen sequence: QDPWAAEPRA ACGPSSCYAL FPRRRTFLEA WRACRELGGD LATPRTPEEA QRVDSLVGAG PASRLLWIGL QRQARQCQLQ RPLRGFTWTT GDQDTAFTNW AQPASGGPCP AQRCVALEAS GEHRWLEGSC TLA; Positive Samples: A-375, Mouse spleen, Mouse thymus; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
CD248 (CD248 Molecule) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include calcium ion binding and extracellular matrix binding. An important paralog of this gene is CD93. CD248 may play a role in tumor angiogenesis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2610111G01Rik; CD164 sialomucin-like 1; CD248; CD248 antigen, endosialin; CD248 molecule, endosialin; Endosialin; MGC119478; MGC119479; Tumor endothelial marker 1
Gene Aliases: 2610111G01Rik; AI842296; CD164L1; CD248; TEM1
UniProt ID: (Human) Q9HCU0, (Mouse) Q91V98
Entrez Gene ID: (Human) 57124, (Mouse) 70445, (Rat) 293669
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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