Invitrogen

CD249 (BP-1) Monoclonal Antibody (6C3), Biotin, eBioscience™

1 Published Figure

12 References

View all (38) CD249 (BP-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD249 (BP-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD249 (BP-1) Antibody (13-5891-81) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.5 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (left) or 0.5 µg of Anti-Mouse BP-1 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Samples were pre-incubated with Anti-Mouse CD16/32 (Purified) (Product # 14-0161-82 to block Fc receptors. Total viable cells were used for analysis.

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Product Details

13-5891-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6C3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466763

Product Specific Information

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.^M
^M
Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis.^M
^M
Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
^M
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Embryonic keratin19⁺ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla.

Nature communications

Lucas B,White AJ,Klein F,Veiga-Villauriz C,Handel A,Bacon A,Cosway EJ,James KD,Parnell SM,Ohigashi I,Takahama Y,Jenkinson WE,Hollander GA,Lu WY,Anderson G

Published figure using CD249 (BP-1) monoclonal antibody (Product # 13-5891-82) in Flow Cytometry

Wed Apr 12 00:00:00 UTC 2023

Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover.

eLife

Xu AQ,Barbosa RR,Calado DP

13-5891 was used in Flow cytometry/Cell sorting to characterize a genetic tool (JchaincreERT2) in the mouse that permits first-ever specific genetic manipulation in PCs in vivo, across immunoglobulin isotypes.

Mon Nov 02 00:00:00 UTC 2020

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Thu Dec 01 00:00:00 UTC 2016

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

13-5891-82 was used in Flow Cytometry to indicate that bone-cartilage-stromal progenitor cells contribute to de novo bone formation during adulthood and may hold substantial regenerative potential.

Wed Jun 01 00:00:00 UTC 2016

BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints.

Developmental biology

Agarwal S,Loder SJ,Brownley C,Eboda O,Peterson JR,Hayano S,Wu B,Zhao B,Kaartinen V,Wong VC,Mishina Y,Levi B

13-5891 was used in Flow cytometry/Cell sorting to characterise a novel mouse model of heterotopic ossification.

Wed Apr 15 00:00:00 UTC 2015

ID4 controls mammary stem cells and marks breast cancers with a stem cell-like phenotype.

Nature communications

Junankar S,Baker LA,Roden DL,Nair R,Elsworth B,Gallego-Ortega D,Lacaze P,Cazet A,Nikolic I,Teo WS,Yang J,McFarland A,Harvey K,Naylor MJ,Lakhani SR,Simpson PT,Raghavendra A,Saunus J,Madore J,Kaplan W,Ormandy C,Millar EK,O'Toole S,Yun K,Swarbrick A

13-5891 was used in Flow cytometry/Cell sorting to investigate the role of ID4 as a regulator of mammary stem cells.

Fri Mar 27 00:00:00 UTC 2015

Stem and progenitor cell division kinetics during postnatal mouse mammary gland development.

Nature communications

Giraddi RR,Shehata M,Gallardo M,Blasco MA,Simons BD,Stingl J

13-5891 was used in Flow cytometry/Cell sorting to investigate the division properties of mammary stem and progenitor cells, showing that most cell division in the adult gland is restricted to the oestrogen receptor-expressing luminal cell lineage.

Thu Oct 29 00:00:00 UTC 2015

Mammary stem cells have myoepithelial cell properties.

Nature cell biology

Prater MD,Petit V,Alasdair Russell I,Giraddi RR,Shehata M,Menon S,Schulte R,Kalajzic I,Rath N,Olson MF,Metzger D,Faraldo MM,Deugnier MA,Glukhova MA,Stingl J

13-5891 was used in Flow cytometry/Cell sorting to show that myoepithelial cells function as long-lived lineage-restricted stem cells in the virgin state and during pregnancy.

Wed Oct 01 00:00:00 UTC 2014

The influence of tamoxifen on normal mouse mammary gland homeostasis.

Breast cancer research : BCR

Shehata M,van Amerongen R,Zeeman AL,Giraddi RR,Stingl J

13-5891 was used in Flow cytometry/Cell sorting to determine if varying doses of tamoxifen skews the distribution and the apoptosis and proliferation status of different types of mammary epithelial cells in vivo.

Thu Jul 24 00:00:00 UTC 2014

Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells.

PLoS biology

Makarem M,Kannan N,Nguyen LV,Knapp DJ,Balani S,Prater MD,Stingl J,Raouf A,Nemirovsky O,Eirew P,Eaves CJ

13-5891 was used in Flow cytometry/Cell sorting to investigate the regenerative potential of foetal mammary epithelial cells compared to adult cells.

Thu Mar 06 00:00:00 UTC 2014

Developmentally regulated availability of RANKL and CD40 ligand reveals distinct mechanisms of fetal and adult cross-talk in the thymus medulla.

Journal of immunology (Baltimore, Md. : 1950)

Desanti GE,Cowan JE,Baik S,Parnell SM,White AJ,Penninger JM,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

13-5891 was used in Flow cytometry/Cell sorting to study how multiple hemopoietic cell types regulate mTEC development through differential provision of RANKL/CD40L during ontogeny, revealing molecular differences in foetal and adult hemopoietic cross-talk.

Sat Dec 15 00:00:00 UTC 2012

PDGFRalpha-expressing mesenchyme regulates thymus growth and the availability of intrathymic niches.

Blood

Jenkinson WE,Rossi SW,Parnell SM,Jenkinson EJ,Anderson G

13-5891 was used in Flow cytometry/Cell sorting to study the regulation of thymus growth and the availability of intrathymic niches by pdgfralpha-expressing mesenchyme.

Thu Feb 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Aminopeptidase A; aminopeptidase-A; AP-A; BP-1/6C3 antigen; BP1; CD249; EAP; Glutamyl aminopeptidase

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Gene Aliases: 6030431M22Rik; APA; Bp-1/6C3; Enpep; Ly-51; Ly51

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UniProt ID: (Mouse) P16406

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Entrez Gene ID: (Mouse) 13809

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Function(s)

aminopeptidase activity peptidase activity metallopeptidase activity zinc ion binding hydrolase activity peptide binding metal ion binding metalloaminopeptidase activity metalloprotease

Process(es)

angiogenesis regulation of systemic arterial blood pressure by renin-angiotensin proteolysis cellular aromatic compound metabolic process cell proliferation cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD249 (BP-1) Monoclonal Antibody (6C3), Biotin, eBioscience™

CD249 (BP-1) Antibody (13-5891-81) in Flow

Product Details

13-5891-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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