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Invitrogen
CD254 (RANK Ligand) Polyclonal Antibody
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FIGURE: 1 / 1
CD254 (RANK Ligand) Antibody (PA5-120439) in WB
Western blot analysis of CD254 (RANK Ligand) in extracts of various cell lines. Samples were incubated with CD254 (RANK Ligand) Polyclonal antibody (Product # PA5-120439) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5 min.
Product Details
PA5-120439
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human RANKL (NP_003692.1).
Conjugate
Form
Liquid
Concentration
0.04 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: K-562, SW620, A-549. The target is usually found in the following locations: Cell membrane, Cytoplasm, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein.
Immunogen sequence: RISEDGTHCI YRILRLHENA DFQDTTLESQ DTKLIPDSCR RIKQAFQGAV QKELQHIVGS QHIRAEKAMV DGSWLDLAKR SKLEAQPFAH LTINATDIPS GSHKVSLSSW YHDRGWAKIS NMTFSNGKLI VNQDGFYYLY ANICFRHHET SGDLATEYLQ LMVYVTKTSI KIPSSHTLMK GGSTKYWSGN SEFHFYSINV GGFFKLRSGE EISIEVSNPS LLDPDQDATY FGAFKVRDID
Target Information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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