Search

  • お問い合わせ一覧​
  • クイックオーダー

Invitrogen

CD254 (RANK Ligand) Polyclonal Antibody

Advanced Verification
1 Published Figure
1 Reference
View all (50) RANKL (RANK Ligand) antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation'] || 'Additional Information'}}: {{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:

{{$ctrl.translations[$ctrl.isSkuNotesCollapsed ? 'antibody.pdp.skuNotes.viewMore' : 'antibody.pdp.skuNotes.viewLess']}}
CD254 (RANK Ligand) Polyclonal Antibody

Product Details

PA5-47641

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1 µg/mL
-

Immunohistochemistry (IHC)

5-15 µg/mL
-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)
-

Neutralization (Neu)

0.02-0.06 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L Arg72-Asp316
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL once reconstituted

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610323

Product Specific Information

In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) CD27, rmCD40, and rmFas is observed and less than 1% cross-reactivity with rmCD30, recombinant human (rh) DR6, rmGITR, rmTNF RI, and rmTNF RII is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H-RANK-L; M-Rank-L; OPG ligand; osteoclast differentiation factor; osteoprotegerin ligand; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TNF-related ligand; tumor necrosis factor-related activation-induced cytokine; unnamed protein product

View more View less

Gene Aliases: Ly109l; ODF; OPGL; RANKL; Trance

View more View less

UniProt ID: (Mouse) O35235

View more View less

Entrez Gene ID: (Mouse) 21943

View more View less

Function(s)
cytokine activity tumor necrosis factor receptor binding protein binding tumor necrosis factor receptor superfamily binding identical protein binding receptor agonist activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification osteoclast proliferation monocyte chemotaxis immune response cell surface receptor signaling pathway organ morphogenesis positive regulation of gene expression cytokine-mediated signaling pathway calcium-mediated signaling osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion osteoclast development paracrine signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade tooth eruption bone resorption regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade lymph node development positive regulation of T cell activation positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis bone development mammary gland alveolus development positive regulation of ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion positive regulation of NIK/NF-kappaB signaling positive regulation of intracellular signal transduction cellular response to leukemia inhibitory factor positive regulation of osteoclast development positive regulation of extrinsic apoptotic signaling pathway
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support