Invitrogen

CD279 (PD-1) Monoclonal Antibody (J116), eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD279 (PD-1) Antibody in Flow Cytometry (Flow) — Staining of human PD-1 transfected cells with anti-human Anti-Human CD279 (PD-1) FITC (left)or PE (right).Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

CD279 (PD-1) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Immunohistochemistry (Frozen)

CD279 (PD-1) Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F)) — Figure 4 Immunohistochemical staining showing the expression of FOXP3, B7-H1 and PD-1 molecules by different subsets of TIL . Representative micrographs at x 530 magnification of (A) double staining of B7-H1 (brown color, membranous/cytoplasmic) and FOXP3 (red color, nuclear) in an area rich in TIL of tumor section. (B) Double staining of PD-1 (brown color, membranous) and FOXP3 (red color, nuclear) in sections from the same tumor as in A. (C) double staining of FOXP3 (brown color, nuclear) and CD8 (red color, membranous). (D) double staining of PD-1 (brown color, membranous) and CD8 (red color, membranous). Solid arrows indicate CD8 + /PD-1 + T lymphocytes and dashed arrow indicates a CD8 - /PD-1 + T lymphocyte. (E) Double staining of B7-H1 (brown color, membranous/cytoplasmic) and CD8 (red color, membranous). Solid arrow indicates a CD8 + /B7-H1 + T lymphocyte and dashed arrow indicates a CD8 - /B7-H1 + T lymphocyte.

CD279 (PD-1) Antibody in Immunohistochemistry (IHC) — Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Immunohistochemistry

Product Details

14-9989-82

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	View 3 publications 3 publications
Flow Cytometry (Flow)	1 µg/test	View 6 publications 6 publications
Immunoprecipitation (IP)	Assay-Dependent	-
Functional Assay (FN)	Assay-Dependent	View 3 publications 3 publications
Inhibition Assays (IA)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Western Blot (WB)	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 2 publications 2 publications
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	J116
Conjugate	Unconjugated Functional Grade
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_468672

Product Specific Information

Description: The J116 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Binding of the J116 monoclonal antibody inhibits PD-1 signal transduction, however, it does not block binding of the ligand PD-L1.

Applications Reported: This J116 antibody has been reported for use in immunoprecipitation and immunohistology staining of frozen tissue sections. It has also been reported in in vitro functional assays. (Please use Functional Grade purified J116, cat. 16-9989, in functional assays.).

Applications Tested: The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Correlations of the expression of γδ T cells and their co-stimulatory molecules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion.

Clinical and experimental immunology

Liang Q,Tong L,Xiang L,Shen S,Pan C,Liu C,Zhang H

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells.

PloS one

Hock BD,MacPherson SA,McKenzie JL

14-9989 was used in Inhibition experiments to raise the possibility that these compounds may provide a useful therapeutic adjunct by reducing immuno-suppression within the tumor micro-environment of CLL.

Tue Aug 22 00:00:00 EDT 2017

PD-L1 expression is associated with advanced non-small cell lung cancer.

Oncology letters

Chen Z,Mei J,Liu L,Wang G,Li Z,Hou J,Zhang Q,You Z,Zhang L

14998982 was used in immunohistochemistry - paraffin section to quantify the expression of programmed cell death protein 1, programmed cell death protein 1 ligand 1, and programmed cell death protein 1 ligand 2 in patients with non-small cell lung cancer

Mon Aug 01 00:00:00 EDT 2016

Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.

Science (New York, N.Y.)

Yoon KW,Byun S,Kwon E,Hwang SY,Chu K,Hiraki M,Jo SH,Weins A,Hakroush S,Cebulla A,Sykes DB,Greka A,Mundel P,Fisher DE,Mandinova A,Lee SW

14-9989 was used in Western Blotting to reveal that p53-induced expression of death domain 1α regulates the efficient clearance of dead cells and proper immune tolerance.

Fri Jul 31 00:00:00 EDT 2015

Immune-escape markers in relation to clinical outcome of advanced melanoma patients following immunotherapy.

Cancer immunology research

Tjin EP,Krebbers G,Meijlink KJ,van de Kasteele W,Rosenberg EH,Sanders J,Nederlof PM,van de Wiel BA,Haanen JB,Melief CJ,Vyth-Dreese FA,Luiten RM

14-9989 was used in Immunohistochemistry on frozen tissues to indicate a more prominent role for T-cell infiltration and activation in tumour tissue than immune-escape mechanisms for clinical outcomes in advanced melanoma patients.

Sun Jun 01 00:00:00 EDT 2014

Inhibiting the programmed death 1 pathway rescues Mycobacterium tuberculosis-specific interferon γ-producing T cells from apoptosis in patients with pulmonary tuberculosis.

The Journal of infectious diseases

Singh A,Mohan A,Dey AB,Mitra DK

14-9989 was used in Flow cytometry/Cell sorting to examine the role of the programmed death 1 pathway in downregulating the T-cell response among patients with pulmonary tuberculosis.

Thu Aug 15 00:00:00 EDT 2013

Cytotoxic activity of dendritic cells as a possible mechanism of negative regulation of T lymphocytes in pulmonary tuberculosis.

Clinical & developmental immunology

Sakhno LV,Tikhonova MA,Tyrinova TV,Leplina OY,Shevela EY,Nikonov SD,Zhdanov OA,Ostanin AA,Chernykh ER

14-9989 was used in Neutralization experiments to investigate PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure in patients with pulmonary tuberculosis.

Thu May 16 00:00:00 EDT 2013

Foxp3+ regulatory T cells among tuberculosis patients: impact on prognosis and restoration of antigen specific IFN-γ producing T cells.

PloS one

Singh A,Dey AB,Mohan A,Sharma PK,Mitra DK

14-9989 was used in Blocking experiments to investigate the impact of Tregs and PD-1 molecules on the severity of human tuberculosis.

Wed Mar 06 00:00:00 EST 2013

IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.

Journal of leukocyte biology

Jurado JO,Pasquinelli V,Alvarez IB,Peña D,Rovetta AI,Tateosian NL,Romeo HE,Musella RM,Palmero D,Chuluyán HE,García VE

14-9989 was used in Flow cytometry/Cell sorting to investigate the induction and regulation of IFN-γ and IL-17 during active human Mycobacterium tuberculosis infection.

Fri Jun 01 00:00:00 EDT 2012

IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.

Journal of leukocyte biology

Jurado JO,Pasquinelli V,Alvarez IB,Peña D,Rovetta AI,Tateosian NL,Romeo HE,Musella RM,Palmero D,Chuluyán HE,García VE

14-9989 was used in Flow cytometry/Cell sorting to investigate the induction and regulation of IFN-γ and IL-17 during active human Mycobacterium tuberculosis infection.

Fri Jun 01 00:00:00 EDT 2012

Immunostaining of PD-1/PD-Ls in liver tissues of patients with hepatitis and hepatocellular carcinoma.

World journal of gastroenterology

Wang BJ,Bao JJ,Wang JZ,Wang Y,Jiang M,Xing MY,Zhang WG,Qi JY,Roggendorf M,Lu MJ,Yang DL

14-9989 was used in Immunohistochemistry to investigate the expression of PD-1, PD-L1 and PD-L2 in liver tissues in the context of chronic hepatitis and hepatocellular carcinoma.

Thu Jul 28 00:00:00 EDT 2011

Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line.

Immunology and cell biology

Otani T,Tsuji-Takayama K,Okochi A,Yamamoto M,Takeuchi M,Yamasaki F,Nakamura S,Kibata M

14-9989 was used in Flow cytometry/Cell sorting to reveal that anti-mouse B7-1 Ab could effectively block IL-10 production, indicating a key role of the B7-1/CD28 pathway.

Tue Feb 01 00:00:00 EST 2011

Expression and function of PDCD1 at the human maternal-fetal interface.

Biology of reproduction

Taglauer ES,Trikhacheva AS,Slusser JG,Petroff MG

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2008

Expression and function of PDCD1 at the human maternal-fetal interface.

Biology of reproduction

Taglauer ES,Trikhacheva AS,Slusser JG,Petroff MG

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2008

Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Jurado JO,Alvarez IB,Pasquinelli V,Martínez GJ,Quiroga MF,Abbate E,Musella RM,Chuluyan HE,García VE

Tue Jul 01 00:00:00 EDT 2008

FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of high-risk breast cancer patients: Implication for immunotherapy.

BMC cancer

Ghebeh H,Barhoush E,Tulbah A,Elkum N,Al-Tweigeri T,Dermime S

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Immunohistochemistry

Sat Feb 23 00:00:00 EST 2008

FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of high-risk breast cancer patients: Implication for immunotherapy.

BMC cancer

Ghebeh H,Barhoush E,Tulbah A,Elkum N,Al-Tweigeri T,Dermime S

Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9989-82) in Immunohistochemistry

Sat Feb 23 00:00:00 EST 2008

Expression of B7-H1 in inflammatory renal tubular epithelial cells.

Nephron. Experimental nephrology

Chen Y,Zhang J,Li J,Zou L,Zhao T,Tang Y,Wu Y

Fri Apr 21 00:00:00 EDT 2006

Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.

Blood

Godfrey WR,Spoden DJ,Ge YG,Baker SR,Liu B,Levine BL,June CH,Blazar BR,Porter SB

Sat Jan 15 00:00:00 EST 2005

Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion.

Nature medicine

Dong H,Strome SE,Salomao DR,Tamura H,Hirano F,Flies DB,Roche PC,Lu J,Zhu G,Tamada K,Lennon VA,Celis E,Chen L

Thu Aug 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: CD279; hPD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

Gene Aliases: CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; SLEB2

UniProt ID: (Human) Q15116

Entrez Gene ID: (Human) 5133

Function(s)

negative regulation of tolerance induction apoptotic process humoral immune response signal transduction multicellular organism development T cell costimulation negative regulation of apoptotic process positive regulation of T cell apoptotic process

Process(es)

signal transducer activity protein binding

CD279 (PD-1) Monoclonal Antibody (J116), eBioscience™

Product Details

14-9989-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (FN)

Inhibition Assays (IA)

Immunohistochemistry (IHC)

Western Blot (WB)

Neutralization (Neu)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!