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Invitrogen
CD2AP Polyclonal Antibody
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FIGURE: 1 / 4
CD2AP Antibody (PA5-96807) in ICC/IF
Immunofluorescence analysis of CD2AP was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD2AP Polyclonal Antibody (Product # PA5-96807) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96807
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 360-639 of human CD2AP (NP_0362521)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.57 mg/mL
Amount
57 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-431, Raji, MCF7, SW480, HL-60, Mouse kidney, Rat kidney; Cellular Location: Cell projection, Cytoplasm, cytoskeleton, ruffle
Immunogen sequence: KYFSLKPEEK DEKSTLEQKP SKPAAPQVPP KKPTPPTKAS NLLRSSGTVY PKRPEKPVPP PPPIAKINGE VSSISSKFET EPVSKLKLDS EQLPLRPKSV DFDSLTVRTS KETDVVNFDD IASSENLLHL TANRPKMPGR RLPGRFNGGH SPTHSPEKIL KLPKEEDSAN LKPSELKKDT CYSPKPSVYL STPSSASKAN TTAFLTPLEI KAKVETDDVK KNSLDELRAQ IIELLCIVEA LKKDHGKELE KLRKDLEEEK TMRSNLEMEI EKLKKAVLSS
Target Information
This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adapter protein CMS; cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology (SH) 3 domains; Cas ligand with multiple Src homology 3 (SH3) domains; CD2AP; mesenchyme to epithelium transition protein, SH3 domains; mesenchyme-to-epithelium transition protein with SH3 domains 1; SH3 domain-containing adapter protein; SH3 domain-containing adaptor protein METS-1
Gene Aliases: CMS; METS-1; Mets1
UniProt ID: (Human) Q9Y5K6, (Mouse) Q9JLQ0, (Rat) F1LRS8
Entrez Gene ID: (Human) 23607, (Mouse) 12488, (Rat) 316258
actin binding
structural constituent of cytoskeleton
protein binding
SH3 domain binding
clathrin binding
protein binding, bridging
phosphatidylinositol 3-kinase regulatory subunit binding
identical protein binding
cadherin binding
actin filament binding
vascular endothelial growth factor receptor binding
beta-catenin binding
macromolecular complex binding
immunological synapse formation
kidney development
liver development
endothelium development
protein phosphorylation
lipid metabolic process
apoptotic process
inflammatory response
response to oxidative stress
actin filament organization
cell proliferation
male gonad development
protein secretion
response to wounding
response to virus
gene expression
protein transport
vesicle organization
cell migration
protein sumoylation
actin filament polymerization
cell differentiation
protein catabolic process
neuron projection development
Rab protein signal transduction
glomerulus development
response to insulin
negative regulation of transforming growth factor beta1 production
regulation of actin cytoskeleton organization
maintenance of blood-brain barrier
neurotrophin signaling pathway
nerve growth factor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
protein kinase B signaling
negative regulation of neuron apoptotic process
cell-cell adhesion mediated by cadherin
cell-cell junction organization
filopodium assembly
neurotrophin TRK receptor signaling pathway
regulation of synaptic plasticity
regulation of receptor-mediated endocytosis
lymph node development
collateral sprouting
positive regulation of protein secretion
synapse organization
T cell receptor signaling pathway
negative regulation of small GTPase mediated signal transduction
protein heterooligomerization
stress-activated MAPK cascade
localization of cell
adipose tissue development
membrane organization
ERK1 and ERK2 cascade
response to transforming growth factor beta
glomerular visceral epithelial cell differentiation
renal albumin absorption
cell-cell adhesion
glucose import across plasma membrane
positive regulation of protein localization to nucleus
response to glial cell derived neurotrophic factor
substrate-dependent cell migration, cell extension
signal transduction
protein-containing complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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