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Invitrogen
CD3/CD19 Antibody Cocktail, FITC, PE
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Product Details
MA5-16537
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Cocktail
Type
Antibody
Clone
6D5/KT3
Immunogen
Human K562 cell line transfected with murine CD19,
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
purified
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-16537 contains a monoclonal mixture of FITC-conjugated CD19 (clone #6D5, Rat IgG2a) and RPE-conjugated CD3 (clone #KT3, Rat IgG2a). This antibody is a dual-color reagent recognizing mouse CD19 and CD3. Clone 6D5 recognizes mouse CD19, a 95 kDa cell surface glycoprotein expressed by B lymphocytes. Clone KT3 recognizes mouse CD3, a cell surface glycoprotein expressed by mature T cells.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-lymphocyte antigen CD19; CD19; CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 epsilon; CD3-zeta/eta; CD3d; CD3e; CD3g; Differentiation antigen CD19; FLJ18683; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain
Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW495831; AW552088; Cd19; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz
UniProt ID: (Mouse) P25918, (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942
Entrez Gene ID: (Mouse) 12478, (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502
chromosome breakage
B cell receptor signaling pathway
positive regulation of release of sequestered calcium ion into cytosol
cell surface receptor signaling pathway
T cell receptor signaling pathway
protein complex assembly
T cell activation
positive thymic T cell selection
positive regulation of T cell anergy
response to nutrient
T cell costimulation
positive regulation of interferon-gamma production
positive regulation of interleukin-4 production
positive regulation of T cell proliferation
negative thymic T cell selection
positive regulation of interleukin-2 biosynthetic process
negative regulation of smoothened signaling pathway
positive regulation of alpha-beta T cell proliferation
lymphocyte activation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
positive regulation of T cell activation
apoptotic signaling pathway
establishment or maintenance of cell polarity
protein transport
regulation of lymphocyte apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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