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Invitrogen

CD3/HLA Class 2 Antibody Cocktail, FITC, PE

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Product Details

MA5-16958

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Neat
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1/IgG2a

Class

Cocktail

Type

Antibody

Clone

UCHT1/WR18

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome, Human B-CLL cells

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538433

Product Specific Information

MA5-16958 contains a monoclonal mixture of FITC-conjugated CD3 (clone #UCH-T1, Mouse IgG1) and RPE-conjugated HLA Class 2 (clone #WR18, Mouse IgG2a). UCHT1 recognizes the human CD3 cell surface antigen expressed by T lymphocytes. WR18 recognizes a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes. This antibody is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes.

Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD74 antigen; cell surface glycoprotein; integral membrane protein; class II HLA histocompatibility antigen; DHLAG; DP beta 1 domain protein; DRB52; FLJ18683; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DQ beta 1 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA DP4 beta-chain; HLA-DP histocompatibility type, beta-1 subunit; HLA-DQ; HLA-DQ-beta-1 cell surface glycoprotein; HLA-DQB1; hla-dr antigen beta chain; HLA-DR-beta chain; HLA-DR-gamma; HLA-DRB1; HLADG; human leucocyte antigen; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; IA-gamma; light chain; lymphocyte antigen DRB1; MHC class II antigen DPB1; MHC class II antigen DQB1; MHC class II antigen HLA-DQ-beta-1; MHC class II DQ beta chain; MHC class II DQ-beta protein precursor; MHC class II DQ3.1ER protein; MHC class II HLA-DP-beta-1; MHC class II HLA-DQ beta glycoprotein; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR4 protein; MHC DQ beta protein precursor; MHC DQ-beta chain; MHC DQw1-beta surface glycoprotein; MHC DR beta protein precursor; MHC DR-beta; MHC DR7-beta-2 chain; MHC HLA DPB1; MHC HLA DR5 cell surface glycoprotein beta chain precursor; MHC HLA-DC-beta chain precursor; MHC HLA-DPw4b-beta chain precursor; MHC HLA-DQ-beta cell surface glycoprotein; MHC HLA-DQ-beta chain precursor old gene name 'HLA-DQB'; MHC HLA-DQ-beta precursor; MHC HLA-DQB1-beta protein; MHC HLA-DR-beta chain precursor; MHC HLA-DR-beta chain precursor old gene name 'HLA-DRA1'; MHC HLA-DR-beta-1 chain; MHC HLA-DR-beta-2 precursor; MHC HLA-DR1-beta; MHC HLA-DR2 (non-Dw2/non-Dw12)b glycoprotein beta-chain; MHC HLA-DR2(Dw12)b glycoprotein beta-chain; MHC HLA-DR2(Dw2)b glycoprotein beta-chain; MHC HLA-DRQ-beta chain; MHC HLA-DRw10-beta-2 protein precursor; MHC HLA-SB-beta chain I; OKT3, delta chain; SB-beta chain; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; unnamed protein product

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Gene Aliases: CD3-DELTA; CD3-GAMMA; CD3D; CD3DELTA; CD3E; CD3epsilon; CD3G; CD3GAMMA; CELIAC1; DPB1; DRB1; HLA-DP; HLA-DP1B; HLA-DPB; HLA-DPB1; HLA-DQB; HLA-DQB1; HLA-DR1B; HLA-DRB; HLA-DRB1; IDDM1; IMD17; IMD18; IMD19; SS1; T3D; T3E; T3G; TCRE

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UniProt ID: (Human) P04234, (Human) P07766, (Human) P09693, (Human) P79608, (Human) P01920, (Human) P01911

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Entrez Gene ID: (Human) 915, (Human) 916, (Human) 917, (Human) 3115, (Human) 3119, (Human) 3123

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Function(s)
transmembrane signaling receptor activity protein binding identical protein binding SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding MHC class II protein complex binding peptide antigen binding MHC class II receptor activity structural constituent of cytoskeleton polysaccharide binding CD4 receptor binding
Process(es)
adaptive immune response immune system process peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of interferon-gamma production positive regulation of T cell proliferation positive regulation of immune response T cell receptor signaling pathway positive regulation of T cell activation cell surface receptor signaling pathway positive thymic T cell selection alpha-beta T cell activation humoral immune response establishment or maintenance of cell polarity protein transport T cell activation gamma-delta T cell activation protein-containing complex assembly regulation of lymphocyte apoptotic process positive regulation of cell-matrix adhesion positive regulation of T cell anergy T cell anergy transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway smoothened signaling pathway regulation of signal transduction positive regulation of gene expression negative regulation of gene expression dendrite development calcium-mediated signaling cerebellum development T cell costimulation positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of cell-cell adhesion mediated by integrin CD4-positive, alpha-beta T cell proliferation T cell proliferation regulation of apoptotic process positive regulation of MAPK cascade negative thymic T cell selection negative regulation of smoothened signaling pathway lymphocyte activation positive regulation of calcium-mediated signaling apoptotic signaling pathway positive regulation of CD4-positive, alpha-beta T cell proliferation positive regulation of T cell mediated cytotoxicity positive regulation of protein phosphorylation inflammatory response to antigenic stimulus myeloid dendritic cell antigen processing and presentation antigen processing and presentation of endogenous peptide antigen via MHC class II positive regulation of T cell mediated immune response to tumor cell negative regulation of inflammatory response to antigenic stimulus cytoskeleton organization signal transduction epidermis development detection of bacterium macrophage differentiation regulation of interleukin-10 production regulation of interleukin-4 production negative regulation of interferon-gamma production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation positive regulation of kinase activity positive regulation of insulin secretion involved in cellular response to glucose stimulus T-helper 1 type immune response negative regulation of T cell proliferation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of memory T cell differentiation regulation of T-helper cell differentiation positive regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of viral entry into host cell protein tetramerization positive regulation of ERK1 and ERK2 cascade positive regulation of CD4-positive, alpha-beta T cell activation
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