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Invitrogen
CD300a Polyclonal Antibody
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FIGURE: 1 / 2
CD300a Antibody (PA5-103096) in ICC/IF
Immunocytochemistry analysis of CD300a in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CD300a polyclonal antibody (Product # PA5-103096) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CD300A(Accession Q9UGN4), corresponding to amino acid residues K221-V271.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CD300A.
Target Information
The CMRF35 antigen (CMRF35A; MIM 606786), which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes. CMRF35H is recognized by the same antibody and is distinct from CMRF35 (Green et al., 1998 [PubMed 9701027]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD300 antigen-like family member A; CD300a; CD300a antigen; CLM-8; CMRF-35-H9; CMRF35-H; CMRF35-H9; CMRF35-like molecule 8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF35HIRp60; IgSF12; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1/IRC2; IRp60; leukocyte membrane antigen; Mcipr1; NK inhibitory receptor; NKRL
Gene Aliases: CD300A; CLM-8; CMRF-35-H9; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF35H; CMRF35H9; HSPC083; IGSF12; IRC1; IRC1/IRC2; IRC2; IRp60
UniProt ID: (Human) Q9UGN4
Entrez Gene ID: (Human) 11314
immune system process
cell adhesion
signal transduction
negative regulation of B cell proliferation
positive regulation of phosphoprotein phosphatase activity
negative regulation of mast cell activation involved in immune response
negative regulation of mast cell degranulation
negative regulation of MAP kinase activity
negative regulation of fibroblast proliferation
regulation of immune response
regulation of T cell receptor signaling pathway
negative regulation of B cell receptor signaling pathway
negative regulation of NK T cell activation
negative regulation of phagocytosis, engulfment
negative regulation of neutrophil activation
negative regulation of eosinophil activation
negative regulation of activation of JAK2 kinase activity
negative regulation of eosinophil migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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