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CD300b Antibody (PA5-99853) in ICC/IF
Immunocytochemistry analysis of CD300b in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CD300b polyclonal antibody (Product # PA5-99853) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99853
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CD300LB(Accession A8K4G0), corresponding to amino acid residues V155-T201.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818786
Product Specific Information
Antibody detects endogenous levels of total CD300LB.
Target Information
CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b; CLM-7; CMRF35-A2; CMRF35-like molecule 7; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; TREM-5; Triggering receptor expressed on myeloid cells 5
Gene Aliases: CD300B; CD300LB; CLM-7; CLM7; CMRF35-A2; CMRF35A2; IREM-3; IREM3; LMIR5; TREM-5; TREM5; UNQ2530/PRO6029
UniProt ID: (Human) A8K4G0
Entrez Gene ID: (Human) 124599
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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