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Invitrogen
CD31 Monoclonal Antibody (HEC7)
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FIGURE: 1 / 28
CD31 Antibody (MA3100) in ICC/IF
Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (HEC7) (Product # MA3100) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antib... View More
Product Details
MA3100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
HEC7
Immunogen
Human PECAM-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3100 targets CD31 in ICC/IF and ACS applications and shows reactivity with Human and Bovine samples.
The MA3100 immunogen is human PECAM-1.
MA3100 detects CD31 which has a predicted molecular weight of approximately 80 kDa.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adhesion molecule; CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; PECAM-1 precursor; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); unnamed protein product
Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1
UniProt ID: (Human) P16284
Entrez Gene ID: (Bovine) 282303, (Human) 5175
positive regulation of protein phosphorylation
angiogenesis
blood vessel development
endothelial cell development
endothelial cell morphogenesis
phagocytosis
immune response
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
leukocyte cell-cell adhesion
signal transduction
cell surface receptor signaling pathway
Rho protein signal transduction
cell recognition
cell migration
regulation of cell migration
positive regulation of cell migration
maintenance of blood-brain barrier
monocyte extravasation
wound healing
vasodilation
positive regulation of MAPK cascade
endothelial cell migration
diapedesis
detection of mechanical stimulus
positive regulation of protein kinase B signaling
establishment of endothelial barrier
bicellular tight junction assembly
cellular response to mechanical stimulus
glomerular endothelium development
neutrophil extravasation
endothelial cell-matrix adhesion
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of protein localization to cell-cell junction
positive regulation of intracellular signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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