Invitrogen

CD31 Monoclonal Antibody (HEC7)

9 Published Figures

22 References

Datasheet

Datasheet

Product Image Gallery

CD31 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD31 in BAEC cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD31 monoclonal antibody (Product # MA3100) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

CD31 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CD31 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD31 monoclonal antibody (Product # MA3100) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

CD31 Antibody — Fig. 4 Vitexin inhibited HI-induced BBB destruction assayed by tight junction-related ZO-1. a TJs are characteristically located at cell-cell contact sites and are intact under physiological conditions. Confocal image of ZO-1 demonstrated disruption of the tight junctions and gap formation following OGD in RBMECs. Arrows indicate tight junction disruption. Scale bar = 50 mum. b Immunofluorescence staining for ZO-1 (red) and CD31 (green), a capillary endothelia marker, in the ischemic cortex of the sham, HI, HI+Bum, and HI+Vit groups 24 h after HI. Merged images of ZO-1 and CD31 staining are also shown. Scale bar = 50 mum. c , d Representative western blot for ZO-1 protein levels in the cerebral cortex from rats of each group. Densitometric value of the protein bands normalized to the respective beta-actin is also shown. * P < 0.05, ** P < 0.01, *** P < 0.001. e The mRNA expression of ZO-1 in the ipsi-ischemic brain tissue of each group was analyzed by real-time quantitative PCR. Data are shown as the mean +- SEM; ** P < 0.01, *** P < 0.001 in comparison with the HI group, n = 4~6 per group, based on a one-way ANOVA

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Published Applications

Immunofluorescence (IF)

See 7 publications below

Western Blot (WB)

See 2 publications below

Immunocytochemistry (ICC)

See 1 publication below

Immunohistochemistry (IHC)

See 3 publications below

Neutralization (Neu)

See 5 publications below

Flow Cytometry (Flow)

See 2 publications below

ELISA (ELISA)

See 1 publication below

Immunoprecipitation (IP)

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Human

Published species

Bovine, Human, Mouse, Rat

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

HEC7

Immunogen

Human PECAM-1

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

RRID

AB_223516

Product Specific Information

MA3100 targets CD31 in ICC/IF and ACS applications and shows reactivity with Human and Bovine samples.

The MA3100 immunogen is human PECAM-1.

MA3100 detects CD31 which has a predicted molecular weight of approximately 80 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1

Gene Aliases: BOS_19340; CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

UniProt ID: (Human) P16284

Entrez Gene ID: (Bovine) 282303, (Human) 5175

Molecular Function: cell adhesion molecule