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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to human CD34 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is angiosarcoma, lung or tonsil tissue.
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tumor endothelium FasL establishes a selective immune barrier promoting tolerance in tumors.
PA1-39457 was used in immunohistochemistry to study the rmechanism by which tumor endothelial FasL promotes tumor immune tolerance
|Motz GT,Santoro SP,Wang LP,Garrabrant T,Lastra RR,Hagemann IS,Lal P,Feldman MD,Benencia F,Coukos G||Nature medicine (20:607)||2014|
CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2