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CD36 Polyclonal Antibody

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CD36 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 18836-1-AP ( CD36 antibody) at dilution of 1:800 (under 40x lens).

CD36 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18836-1-AP (CD36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CD36 Antibody in Flow Cytometry (Flow) — 1X10^6 RAW 264.7 cells were stained with 0.2ug CD36 antibody (18836-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

18836-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,500	View 20 publications 20 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:400-1:1,000	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cytotoxic Necrotizing Factor 1 Downregulates CD36 Transcription in Macrophages to Induce Inflammation During Acute Urinary Tract Infections.

Frontiers in immunology

Yang H,Li Q,Wang C,Wang J,Lv J,Wang L,Zhang ZS,Yao Z,Wang Q

Mon Sep 30 00:00:00 EDT 2019

Cytotoxic Necrotizing Factor 1 Downregulates CD36 Transcription in Macrophages to Induce Inflammation During Acute Urinary Tract Infections.

Frontiers in immunology

Yang H,Li Q,Wang C,Wang J,Lv J,Wang L,Zhang ZS,Yao Z,Wang Q

Mon Sep 30 00:00:00 EDT 2019

Novel metabolic disorders in skeletal muscle of Lipodystrophic Bscl2/Seipin deficient mice.

Molecular and cellular endocrinology

Xu W,Zhou H,Xuan H,Saha P,Wang G,Chen W

Fri Feb 15 00:00:00 EST 2019

CD36 plays a critical role in proliferation, migration and tamoxifen-inhibited growth of ER-positive breast cancer cells.

Oncogenesis

Liang Y,Han H,Liu L,Duan Y,Yang X,Ma C,Zhu Y,Han J,Li X,Chen Y

Fri Dec 21 00:00:00 EST 2018

CD36 plays a critical role in proliferation, migration and tamoxifen-inhibited growth of ER-positive breast cancer cells.

Oncogenesis

Liang Y,Han H,Liu L,Duan Y,Yang X,Ma C,Zhu Y,Han J,Li X,Chen Y

Fri Dec 21 00:00:00 EST 2018

Isoquercitrin Attenuated Cardiac Dysfunction Via AMPKα-Dependent Pathways in LPS-Treated Mice.

Molecular nutrition & food research

Huang SH,Xu M,Wu HM,Wan CX,Wang HB,Wu QQ,Liao HH,Deng W,Tang QZ

Sat Dec 01 00:00:00 EST 2018

The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.

The Journal of biological chemistry

Thapa D,Wu K,Stoner MW,Xie B,Zhang M,Manning JR,Lu Z,Li JH,Chen Y,Gucek M,Playford MP,Mehta NN,Harmon D,O'Doherty RM,Jurczak MJ,Sack MN,Scott I

Fri Nov 16 00:00:00 EST 2018

Air pollution is associated with the development of atherosclerosis via the cooperation of CD36 and NLRP3 inflammasome in ApoE^-/- mice.

Toxicology letters

Du X,Jiang S,Zeng X,Zhang J,Pan K,Zhou J,Xie Y,Kan H,Song W,Sun Q,Zhao J

Fri Jun 15 00:00:00 EDT 2018

Cereal Fiber Ameliorates High-Fat/Cholesterol-Diet-Induced Atherosclerosis by Modulating the NLRP3 Inflammasome Pathway in ApoE^-/- Mice.

Journal of agricultural and food chemistry

Zhang R,Han S,Zhang Z,Zhang W,Yang J,Wan Z,Qin L

Wed May 16 00:00:00 EDT 2018

Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein.

Technology in cancer research & treatment

Wang LK,Xie XN,Song XH,Su T,Chang XL,Xu M,Liang B,Huang DY

Mon Jan 01 00:00:00 EST 2018

Proatherogenic Flow Increases Endothelial Stiffness via Enhanced CD36-Mediated Uptake of Oxidized Low-Density Lipoproteins.

Arteriosclerosis, thrombosis, and vascular biology

Le Master E,Huang RT,Zhang C,Bogachkov Y,Coles C,Shentu TP,Sheng Y,Fancher IS,Ng C,Christoforidis T,Subbaiah PV,Berdyshev E,Qain Z,Eddington DT,Lee J,Cho M,Fang Y,Minshall RD,Levitan I

Mon Jan 01 00:00:00 EST 2018

Proatherogenic Flow Increases Endothelial Stiffness via Enhanced CD36-Mediated Uptake of Oxidized Low-Density Lipoproteins.

Arteriosclerosis, thrombosis, and vascular biology

Le Master E,Huang RT,Zhang C,Bogachkov Y,Coles C,Shentu TP,Sheng Y,Fancher IS,Ng C,Christoforidis T,Subbaiah PV,Berdyshev E,Qain Z,Eddington DT,Lee J,Cho M,Fang Y,Minshall RD,Levitan I

Mon Jan 01 00:00:00 EST 2018

CD36 Deficiency Suppresses Epileptic Seizures.

Neuroscience

Zheng F,Yang Y,Lu S,Yang Q,Li Y,Xu X,Zhang Y,Liu F,Tian X,Wang X

Tue Dec 26 00:00:00 EST 2017

CD36 Deficiency Suppresses Epileptic Seizures.

Neuroscience

Zheng F,Yang Y,Lu S,Yang Q,Li Y,Xu X,Zhang Y,Liu F,Tian X,Wang X

Tue Dec 26 00:00:00 EST 2017

Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet.

PloS one

Zhang M,Zhao H,Cai J,Li H,Wu Q,Qiao T,Li K

Fri Nov 03 00:00:00 EDT 2017

Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet.

PloS one

Zhang M,Zhao H,Cai J,Li H,Wu Q,Qiao T,Li K

Fri Nov 03 00:00:00 EDT 2017

Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.

PloS one

Hu YW,Ma X,Huang JL,Mao XR,Yang JY,Zhao JY,Li SF,Qiu YR,Yang J,Zheng L,Wang Q

Thu Oct 12 00:00:00 EDT 2017

Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.

Archives of medical science : AMS

Liu F,Wang Y,Xu J,Liu F,Hu R,Deng H

Sat Oct 01 00:00:00 EDT 2016

Inhibition of Macrophage CD36 Expression and Cellular Oxidized Low Density Lipoprotein (oxLDL) Accumulation by Tamoxifen: A PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR (PPAR)γ-DEPENDENT MECHANISM.

The Journal of biological chemistry

Yu M,Jiang M,Chen Y,Zhang S,Zhang W,Yang X,Li X,Li Y,Duan S,Han J,Duan Y

Fri Aug 12 00:00:00 EDT 2016

VNN1 promotes atherosclerosis progression in apoE-/- mice fed a high-fat/high-cholesterol diet.

Journal of lipid research

Hu YW,Wu SG,Zhao JJ,Ma X,Lu JB,Xiu JC,Zhang Y,Huang C,Qiu YR,Sha YH,Gao JJ,Wang YC,Li SF,Zhao JY,Zheng L,Wang Q

Mon Aug 01 00:00:00 EDT 2016

Blood-brain barrier and blood-cerebrospinal fluid barrier in normal and pathological conditions.

Brain tumor pathology

Ueno M,Chiba Y,Murakami R,Matsumoto K,Kawauchi M,Fujihara R

Fri Apr 01 00:00:00 EDT 2016

Impact of age and sex on the development of atherosclerosis and expression of the related genes in apoE deficient mice.

Biochemical and biophysical research communications

Liu M,Zhang W,Li X,Han J,Chen Y,Duan Y

Fri Jan 15 00:00:00 EST 2016

Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells.

Molecules (Basel, Switzerland)

Hao S,Ji J,Zhao H,Shang L,Wu J,Li H,Qiao T,Li K

Tue Dec 01 00:00:00 EST 2015

Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood-cerebrospinal fluid barrier in human brain.

Histochemistry and cell biology

Matsumoto K,Chiba Y,Fujihara R,Kubo H,Sakamoto H,Ueno M

Tue Dec 01 00:00:00 EST 2015

LXRalpha gene downregulation by lentiviral-based RNA interference enhances liver function after fatty liver transplantation in rats.

Hepatobiliary & pancreatic diseases international : HBPD INT

Zhao YP,Li L,Ma JP,Chen G,Bai JH

Sat Aug 01 00:00:00 EDT 2015

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction.

Arteriosclerosis, thrombosis, and vascular biology

Hu YW,Zhao JY,Li SF,Huang JL,Qiu YR,Ma X,Wu SG,Chen ZP,Hu YR,Yang JY,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Thu Jan 01 00:00:00 EST 2015

Orally active osteoanabolic agent GTDF binds to adiponectin receptors, with a preference for AdipoR1, induces adiponectin-associated signaling, and improves metabolic health in a rodent model of diabetes.

Diabetes

Singh AK,Joharapurkar AA,Khan MP,Mishra JS,Singh N,Yadav M,Hossain Z,Khan K,Kumar S,Dhanesha NA,Mishra DP,Maurya R,Sharma S,Jain MR,Trivedi AK,Godbole MM,Gayen JR,Chattopadhyay N,Sanyal S

Wed Oct 01 00:00:00 EDT 2014

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis.

Journal of lipid research

Hu YW,Yang JY,Ma X,Chen ZP,Hu YR,Zhao JY,Li SF,Qiu YR,Lu JB,Wang YC,Gao JJ,Sha YH,Zheng L,Wang Q

Tue Apr 01 00:00:00 EDT 2014

CD36 expression in the brains of SAMP8.

Archives of gerontology and geriatrics

Wu B,Ueno M,Kusaka T,Miki T,Nagai Y,Nakagawa T,Kanenishi K,Hosomi N,Sakamoto H

Fri May 03 00:00:00 EDT 2013

CD36 expression in the brains of SAMP8.

Archives of gerontology and geriatrics

Wu B,Ueno M,Kusaka T,Miki T,Nagai Y,Nakagawa T,Kanenishi K,Hosomi N,Sakamoto H

Fri May 03 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor

Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3

UniProt ID: (Human) P16671

Entrez Gene ID: (Human) 948

Molecular Function: membrane trafficking regulatory protein

Function(s)

Process(es)

low-density lipoprotein receptor activity protein binding high-density lipoprotein particle binding lipid binding low-density lipoprotein particle binding transforming growth factor beta binding thrombospondin receptor activity lipoteichoic acid receptor activity lipoprotein particle binding

CD36 Polyclonal Antibody

Product Details

18836-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Target Information

Bioinformatics

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