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          • Primary Antibodies ›
          • CD36 Antibodies

          Invitrogen

          CD36 Polyclonal Antibody, HRP

          1 Published Figure
          1 Reference
          View all (73) CD36 antibodies

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          Cite CD36 Polyclonal Antibody, HRP

          Additional Information:
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          • Published Figures (1)
          CD36 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          CD36 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          CD36 Antibody (PA5-33291) in WB

          Nature communications 2022 - Fig. 6 CD1d regulates CD36 internalization and lipid uptake. a qPCR expression data of Cd36 and Msr1 in WT and CD1d-KO cells ( n = 4-6). b Western-blots and quantification for CD36 and beta-actin in WT and CD1d-KO BMDCs ( n = 3). c Flow cytometry images and quantification of Mean fluorescence intensity (MFI) for CD36 and MSR1 in WT and CD1d-KO cells ( n = 3-13). * p < 0.05, *** p < 0.001, one-sample t -test... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CD36 Antibody in Western Blot (WB)
          CD36 Polyclonal Antibody, HRP

          Product Details

          PA5-33291

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-Dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-Dependent
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Avian, Bovine, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide mapping to a region of human CD36 between residues 100-200.
          View immunogen

          Conjugate

          HRP HRP HRP

          Form

          Liquid

          Concentration

          0.78 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2550675

          Product Specific Information

          Western blot shows a band at approx.

          75-80 kDa.

          The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein.Suggested positive control: antigen standard for CD36 (transient overexpression lysate).

          Target Information

          CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 85 kda protein; 88 kda cell surface glycoprotein; adipocyte membrane protein; CD36; CD36 antigen; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (CD36 blood group) transcript; CD36 molecule (thrombospondin receptor); CD36 molecule (thrombospondin receptor) isoform CD36_1; CD36 molecule (thrombospondin receptor) isoform CD36_2; CD36 molecule (thrombospondin receptor) isoform CD36_3; cluster determinant 36; collagen type I receptor thrombospondin receptor; collagen type I receptor, thrombospondin receptor; collagen type I receptor; thrombospondin receptor; FAT; FAT/CD36; Fatty acid translocase; fatty acid translocase/CD36; fatty acid transport protein; glycoprotein GPIIIb/GPIV; Glycoprotein IIIb; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scavenger receptor class B, member 3; SR-B3; Thrombospondin receptor; unnamed protein product

          View more View less

          Gene Aliases: BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIIIB; GPIV; PAS-4; PAS4; PASIV; SCARB3

          View more View less

          UniProt ID: (Bovine) P26201, (Human) P16671, (Mouse) Q08857

          View more View less

          Entrez Gene ID: (Bovine) 281052, (Pig) 733702, (Human) 948, (Rabbit) 100008825, (Rat) 29184, (Mouse) 12491

          View more View less

          Function(s)
          beta-amyloid binding low-density lipoprotein receptor activity scavenger receptor activity long-chain fatty acid transporter activity protein binding high-density lipoprotein particle binding lipid binding short-chain fatty acid uptake transporter activity low-density lipoprotein particle binding Toll-like receptor binding cargo receptor activity macromolecular complex binding transforming growth factor beta binding thrombospondin receptor activity lipoteichoic acid receptor activity lipoprotein particle binding oxidised low-density lipoprotein particle receptor activity oleate transmembrane transporter activity oleic acid binding membrane trafficking regulatory protein
          Process(es)
          immune response cell adhesion cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration intestinal cholesterol absorption receptor internalization interleukin-1 beta production response to lipid triglyceride transport long-chain fatty acid import intestinal absorption sensory perception of taste positive regulation of ERK1 and ERK2 cascade cholesterol import response to fatty acid response to linoleic acid cellular response to low-density lipoprotein particle stimulus cellular response to diacyl bacterial lipopeptide energy homeostasis positive regulation of NLRP3 inflammasome complex assembly amyloid fibril formation negative regulation of transcription from RNA polymerase II promoter MAPK cascade positive regulation of cell-matrix adhesion production of molecular mediator involved in inflammatory response lipid metabolic process fatty acid metabolic process lipid transport receptor-mediated endocytosis phagocytosis, recognition phagocytosis, engulfment blood coagulation response to bacterium positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol storage fatty acid transport long-chain fatty acid transport plasma membrane long-chain fatty acid transport short-chain fatty acid transport negative regulation of angiogenesis lipid storage cGMP-mediated signaling positive regulation of blood coagulation cholesterol transport regulation of lipopolysaccharide-mediated signaling pathway positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production regulation of toll-like receptor signaling pathway plasma lipoprotein particle clearance low-density lipoprotein particle clearance cellular response to oxidative stress response to stilbenoid nitric oxide-cGMP-mediated signaling pathway negative regulation of protein import into nucleus lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly apoptotic cell clearance positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis defense response to Gram-positive bacterium low-density lipoprotein particle mediated signaling positive regulation of phagocytosis, engulfment positive regulation of macrophage cytokine production cellular response to bacterial lipopeptide cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to hydroperoxide regulation of action potential positive regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus oxidised low-density lipoprotein particle clearance amyloid-beta clearance by cellular catabolic process positive regulation of protein localization to plasma membrane positive regulation of reactive oxygen species biosynthetic process cellular response to beta-amyloid lipid transport across blood brain barrier regulation of removal of superoxide radicals positive regulation of blood microparticle formation positive regulation of reactive oxygen species metabolic process long-chain fatty acid metabolic process pattern recognition receptor signaling pathway negative regulation of systemic arterial blood pressure triglyceride metabolic process signal transduction nitric oxide mediated signal transduction response to nutrient response to mechanical stimulus response to activity fatty acid oxidation response to estradiol cellular response to insulin stimulus response to drug negative regulation of transcription factor import into nucleus negative regulation of growth of symbiont in host digestive tract development positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation response to growth hormone transport regulation of transcription from RNA polymerase II promoter in response to oxidative stress
          It has to be done as per old AB suggested Products section.

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