Proteintech

CD39/ENTPD1 Polyclonal Antibody

Product Image Gallery

CD39/ENTPD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 10x lens).

CD39/ENTPD1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CD39/ENTPD1 Antibody in Flow Cytometry (Flow) — 1X10^6 Jurkat cells were stained with 0.5ug CD39 antibody (19229-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

CD39/ENTPD1 Antibody in Immunoprecipitation (IP) — IP result of anti-CD39 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

Published Applications

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CD39/ENTPD1 Fusion Protein Ag6165

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478 aa) molecule.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATPDase; CD39; CD39 antigen; Ecto apyrase; Ecto ATP diphosphohydrolase 1; Ecto ATPase 1; Ecto ATPDase 1; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; Lymphoid cell activation antigen; NTPDase 1

Gene Aliases: 2610206B08Rik; AA408691; ATPDase; CD39; ENTPD1; NTPDase-1; SPG64

UniProt ID: (Human) P49961, (Mouse) P55772

Entrez Gene ID: (Human) 953, (Mouse) 12495, (Rat) 64519

Molecular Function: hydrolase nucleotide phosphatase phosphatase