Proteintech

CD39/ENTPD1 Polyclonal Antibody

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CD39/ENTPD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 40x lens).

CD39/ENTPD1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CD39/ENTPD1 Antibody in Flow Cytometry (Flow) — 1X10^6 Jurkat cells were stained with 0.5ug CD39 antibody (19229-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

CD39/ENTPD1 Antibody in Immunoprecipitation (IP) — IP result of anti-CD39 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.

Product Details

19229-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Flow Cytometry (Flow)	0.5 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD39/ENTPD1 Fusion Protein Ag6165 (1-340 aa encoded by BC047664) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478 aa) molecule.

Immunogen sequence: MESEELADR VLDVVERSLS NYPFDFQGAR IITGQEEGAY GWITINYLLG KFSQKTRWFS IVPYETNNQE TFGALDLGGA STQVTFVPQN QTIESPDNAL QFRLYGKDYN VYTHSFLCYG KDQALWQKLA KDIQVASNEI LRDPCFHPGY KKVVNVSDLY KTPCTKRFEM TLPFQQFEIQ GIGNYQQCHQ SILELFNTSY CPYSQCAFNG IFLPPLQGDF GAFSAFYFVM KFLNLTSEKV SQEKVTEMMK KFCAQPWEEI KTSYAGVKEK YLSEYCFSGT YILSLLLQGY HFTADSWEHI HFIGKIQGSD AGWTLGYMLN LTNMIPAEQP LSTPLSHSTY V (1-340 aa encoded by BC047664)

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.

Bone research

Liu W,Zhang L,Xuan K,Hu C,Liu S,Liao L,Li B,Jin F,Shi S,Jin Y

Wed Jul 28 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: ATPDase; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1

Gene Aliases: 2610206B08Rik; AA408691; ATPDase; CD39; ENTPD1; NTPDase-1; SPG64

UniProt ID: (Human) P49961, (Mouse) P55772

Entrez Gene ID: (Human) 953, (Mouse) 12495, (Rat) 64519

Molecular Function: hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme