Bioss

CD3E Polyclonal Antibody

View all (466) CD3 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

BS-10498R

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Flow Cytometry (Flow)

1:20-1:100
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from pig CD3E.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD247 antigen, zeta subunit; CD3 antigen delta chain; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon subunit; CD3 TCR complex; CD3 zeta chain; CD3-epsilon; CD3-zeta/eta; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TcR CD3 delta-chain; TCR zeta chain

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; CD247; CD3; CD3-DELTA; Cd3-eta; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3epsilon; CD3G; CD3H; CD3Q; CD3Z; Cd3zeta; Ctg-3; Ctg3; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; Tcrk; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Pig) Q764N2, (Human) P07766, (Pig) Q7YRN2, (Pig) Q5PXD3, (Human) P09693, (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Pig) 397302, (Human) 919, (Human) 915, (Pig) 396661, (Human) 916, (Pig) 397455, (Pig) 494013, (Human) 917, (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)
transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding signal transducer activity defense/immunity protein immunoglobulin receptor superfamily
Process(es)
adaptive immune response viral process T cell costimulation Fc-gamma receptor signaling pathway involved in phagocytosis regulation of defense response to virus by virus regulation of immune response T cell receptor signaling pathway cell surface receptor signaling pathway T cell differentiation positive thymic T cell selection positive regulation of transcription from RNA polymerase II promoter positive regulation of T cell anergy protein complex assembly transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway response to nutrient positive regulation of gene expression negative regulation of gene expression positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation T cell activation regulation of apoptotic process negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling apoptotic signaling pathway establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process lymphocyte activation positive regulation of T cell activation
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