This antibody reacts with the intracytoplasmic portion of CD3 and stains human T cells in both the cortex and medula of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin embedded tissues. For tissue staining, antigen retrieval is suggested by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
This antibody is predicted to react with Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Baboon, and Woodchuck.
Recommended positive controls:
WB - Recombinant human CD3e protein, Jurkat whole cell lysate, Human, mouse and rat thymus tissue lysate|
IHC (P) - Pig and rat spleen tissue, human tonsil tissue, mouse epidydimal fat pad and lymph node tissues, rat infarcted heart and spleen tissues
Flow Cytometry - Human peripheral blood lymphocytes, Jurkat cells
IHC (F) - Mouse brain tissue
The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD247; CD247 antigen; CD247 antigen, zeta subunit; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon subunit; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3 zeta chain; CD3-epsilon; CD3-zeta/eta; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TcR CD3 delta-chain; TCR zeta chain
Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; CD247; CD3; CD3-DELTA; Cd3-eta; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3epsilon; CD3G; CD3H; CD3Q; CD3Z; Cd3zeta; Ctg-3; Ctg3; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; Tcrk; TCRZ; TCRzeta
UniProt ID: (Human) P20963, (Pig) Q764N2, (Human) P04234, (Human) P07766, (Pig) Q7YRN2, (Human) P09693, (Pig) Q5PXD3, (Mouse) P24161, (Mouse) P04235, (Rat) P19377, (Mouse) P22646, (Mouse) P11942, (Rat) Q64159
Entrez Gene ID: (Human) 919, (Pig) 397302, (Pig) 396661, (Human) 915, (Human) 916, (Pig) 397455, (Human) 917, (Pig) 494013, (Rat) 25300, (Mouse) 12503, (Mouse) 12500, (Rat) 25710, (Rat) 315609, (Mouse) 12501, (Mouse) 12502, (Rat) 300678