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Invitrogen
CD3e Monoclonal Antibody (SP7)
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CD3e Antibody (MA5-14524) in IHC (P)
Immunohistochemical analysis of CD3e was performed using formalin-fixed paraffin-embedded mouse thymus and mouse brain tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 1... View More
Product Details
MA5-14524
Applications
Tested Dilution
Publications
Product Specifications
Published species
C. elegans,
Dog,
Human,
Mouse,
Non-human primate,
Rat,
Rhesus monkey
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP7
Immunogen
A synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon-chain of human CD3 protein
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-14524 targets CD3E in IHC (P), FACS, and Western blot applications and shows reactivity with human and mouse samples.
The MA5-14524 immunogen is a synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon-chain of human CD3 protein.
Target Information
The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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