|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human thymocytes followed by Sezary T cells|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||20 ul/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disease-specific and inflammation-independent stromal alterations in spondylarthritis synovitis.
MA1-10179 was used in immunohistochemistry to identify novel disease-specific pathways in spondylarthritis synovitis using geneome-wide microarray analysis
|Yeremenko N,Noordenbos T,Cantaert T,van Tok M,van de Sande M,Cañete JD,Tak PP,Baeten D||Arthritis and rheumatism (65:174)||2013|
B cell antigen receptor signaling enhances IFN-gamma-induced Stat1 target gene expression through calcium mobilization and activation of multiple serine kinase pathways.
MA1-10179 was used in flow cytometry to study the mechanism by which B cell antigen receptor signaling enhances IFN-gamma-induced Stat1 target gene expression
|Xu W,Nair JS,Malhotra A,Zhang JJ||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (25:113)||2005|